An Improved Method of Protoplast Regeneration for Bacillus Species and Its Application to Protoplast Fusion and Transformation
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概要
- 論文の詳細を見る
An improved method for regenerating Bacillus subtilis protoplasts at the frequency of 92-100% on a semi-synth tic medium was found. Protoplasts were preincubated in HCP-3 medium, an isotonic semi-synthetic medium supplemented with polyvinylpyrrolidone, and then plated on HCP-1.5 agar medium by overlaying. By this method, even on the regeneration medium supplemented with minimal nutritional requirements protoplasts regenerated at a frequency of as high as 20%. The modified method was applicable to the direct-selection of prototrophic recombinants after fusion (the highest recombination yield from the input protoplasts was 1.3%) and to protoplast transformation with plasmid DNA.
- 社団法人 日本農芸化学会の論文
著者
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Akamatsu Takashi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Sekiguchi Junichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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SEKIGUCHI Junichi
Department of Applied Microbiology and Technology, Kumamoto Institute of Technology
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