A simple assay for 6-aminohexanoate-oligomer-hydrolase using N-(4-nitrophenyl)-6-aminohexanamide(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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We synthesized N-(4-nitrophenyl)-6-aminohexanamide (AHpNA) and used it as a substrate in a kinetic study of 6-aminohexanoate-hydrolase (NylB), a nylon oligomer-hydrolyzing enzyme. NylBs derived from Arthrobacter sp. KI72 and Pseudomonas sp. NK87 hydrolyzed AHpNA as well as a 6-aminohexanoic acid dimer, a known substrate for NylB. The K_m values of the NylB from Arthrobacter sp. KI72 and Pseudomonas sp. NK87 for AHpNA were 0.5 mM and 2.0 mM, respectively.
- 2012-12-00
著者
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Akamatsu Takashi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Shin Takashi
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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ONO Shin
Graduate School of Science and Engineering, University of Toyama
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Taguchi Hisataka
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Ono Shin
Graduate School Of Science And Engineering University Of Toyama
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Wakamatsu Makoto
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University
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Aso Kenji
Graduate School of Science and Engineering, University of Toyama
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Shin Takashi
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University
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SHIN Takashi
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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