A Simple and Rapid Method for Intra-and Interspecific Transformation of Bacillus subtilis on Solid Media by DNA in Protoplast Lysates
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概要
- 論文の詳細を見る
A simple method for intra-and interspecific transformation of Bacillus subtilis on solid media has been devised with DNA in protoplast lysates, 0.8% agar, glutamate, and yeast extract. The transformation frequency is 2.3×10^3 transformants per μg DNA, 10-20 times higher than that for conventional transformation on solid media.^<1)> The method can be applicable to transformation in microtiter plates.
- 社団法人日本農芸化学会の論文
- 2001-02-23
著者
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Taguchi Hisataka
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Akamatsu Takashi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Akamatsu Takashi
Department Of Applied Microbial Technology Sojo University
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Taguchi Hisataka
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Taguchi Hisataka
Department Of Applied Microbial Technology Sojo University
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