Colorimetric Measurement of Angiotensin I-Converting Enzyme Activity Using Bilirubin Oxidase-Catalyzed Oxidative Decarboxylation

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概要

• 論文の詳細を見る

• A simple colorimetric method for determining the activity of angiotensin I-converting enzyme (ACE) was developed. The assay method is based on the following series of reactions : ACE hydrolyzes a tripeptide substrate, N-benzoyl-L-2-(4-hydroxyphenyl)glycyl-L-histidyl-_L-leucine to liberate N-benzoyl L-2-(4-hydroxyphenyl)glycine. The compound is then converted into 4-hydroxybenzaldehyde (HBA) through oxidative decarboxylation catalyzed by bilirubin oxIdase from Myrothecium verrucaria. Finally, HBA is measured colorimetrically by treatment with 2,4-dinitrophenylhydrazine to evaluate the activity of ACE. The optimum pH of ACE for the substrate was 8.3,and the K_m value aws 0.072mM. The formation rate of HBA was proportional to the ACE concentration, and the correlation coefficient was 0.997.

• 社団法人日本生物工学会の論文
• 1994-01-25

著者

• SHIN Takashi

  Department of Applied Microbial Technology, The Kumamoto Institute of Technology

• CHIBA Hiroyuki

  Process Development Laboratories, Mercian Corporation

• AGEMATU Hitosi

  Central Research Laboratories, Mercian Corporation

• Chiba Hiroyuki

  Central Research Laboratories Mercian Corporation

• Shin T

  Department Of Applied Microbial Technology The Kumamoto Institute Of Technology

• Shin Takashi

  Department Of Applied Microbial Technology Kumamoto Institute Of Technology

• Agematu Hitosi

  Central Research Laboratories Mercian Corporation

• Yoshioka Takeo

  Central Research Laboratories Mercian Corporation

• Shibamoto Norio

  Central Research Laboratories Mercian Co.

• Chiba Hiroyuki

  Process Development Laboratories Mercian Corporation

• Agematu Hitosi

  Central Research Laboratories Mercian Co.

• Agematu H

  Mercian Corp. Fujisawa Jpn

• Takenisi Shigeyuki

  Osaka Municipal Technical Research Institute

• Yoshioka Takeo

  Central Research Laboratories, Mercian Corporation

• SHIN Takashi

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture

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