Isolation and Growth Characteristics of Nitrilotriacetate-Degrading Bacteria
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概要
- 論文の詳細を見る
Two bacterial strains that degrade nitrilotriacetate (NTA) were isolated from NTA-acclimatized activated sludge. These bacteria grew well in NTA medium with optimal pH around 7. The growth rate constants of the bacteria, strains N-2 and N-5,were 0.046 h^<-1> and 0.11 h^<-1> at the concentration of 0.1% NTA (pH 7.0,25℃), respectively. The growth of each bacterium was inhibited at high concentrations of NTA. The growth rate decreased roughly linearly with increasing concentration of NTA. The strains N-2 and N-5 showed maximal cell growth at the concentrations of 0.2% and 0.25% NTA, respectively. The strain N-2 would not grow at the concentration of 0.5% NTA. On the other hand, the strain N-5 showed a little growth under the same conditions. Also, the bacterial growth was almost completely inhibited when divalent metal ions such as Mg^<++>, Ca^<++>, and Fe^<++> were omitted from the culture medium, or slightly excess EDTA (1 mM) was added to the medium. These results suggest that the bacterial growth inhibition at high concentration of NTA is caused by the sequestration of metal ions in the medium.
- 社団法人日本生物工学会の論文
- 1986-04-25
著者
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SHIMIZU Tokuo
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University
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Yamaguchi Hiroyuki
Department of Internal Medicine, Yamagata Prefectural Shinjo Hospital
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SHIRAKASHI Takashi
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University
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Yamaguchi Hiroyuki
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Shirakashi Takashi
Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University
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KAKII KAZUO
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University
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KURIYAMA MITSUO
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University
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IGUCHI YUTAKA
Department of Environmental Chemistry, Faculty of Engineering, Utsunomiya University
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TESHIMA MUNEHIRO
Department of Environmental Chemistry, Faculty of Engineering, Utsunomiya University
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Shimizu T
Hakodate National Coll. Technol. Hakodate
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Kuriyama Mitsuo
Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University
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Tamura M
Utsunomiya Univ. Utsunomiya Jpn
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Kakii K
Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University
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Kakii Kazuo
Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University
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Kuriyama M
Utsunomiya Univ. Utsunomiya Jpn
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Yamaguchi Hiroyuki
Department Of Environmental Chemistry Faculty Of Engineering Utsunomiya University
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