Some Properties of Mucilage Polysaccharides Extracted from Sewage Activated Sludge

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Mucilage polysaccharides were hydrolyzed under nitrogen at 100℃ for 12-72 h in 0.05 N H_2SO_4 in the presence of Dowex 50W(H^+ form). Maximial values of released sugars were reached after 48 h, with very little destruction with prolonged hydrolysis periods. The following sugars were detected and the molar ration of these sugars to glucose is shown in the parentheses. Glucose (1.00), galactose (1.00), mannose (0.75), rhamnose (0.64), fucose (0.47), arabinose (0.32), xylose (0.25), and glucuronic acid (0.49). Although ribose was detected as a component, it was liberated from ribonucleic acid contained in the mucilage, but not from the polysaccharide fraction.Mucilage polysaccharides were divided into three fractions by ion exchange chromatography using diethylaminoethyl (DEAE)-cellulose. The non-adsorbed fraction occupied only 7% of total sugars. 74% of total sugars was recovered by elution with a linear gradient of NaCl and with 0.1 N NaOH. The remaining 20% was irreversibly adsorbed to the column, and not recovered. Gel filtration on Sepharose CL-6B indicated that these polysaccharides might have a macromolecular structure.From these results, it is concluded that the mucilage polysaccharides extracted from sewage activated sludge are acidic heteropolysaccharides which contain glucuronic acid.
社団法人日本生物工学会の論文
1984-10-25