Isolation and Characterization of a Ca^<++>-Dependent Floc-Forming Bacterium

概要

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When sewage activated sludge was treated with a commercial proteolytic enzyme, Actinase E, 12% of the sludge was deflocculated. However, most of the deflocculated sludge was sedimented by low-speed centrifugation, indicating that only a small part of the sludge was deflocculated to the level of free cells. When bacteria were isolated using the supernatant cell suspension obtained by Actinase treatment and the subsequent low-speed centrifugation, two Actinase E-susceptible floc-forming bacteria were isolated.In this study, flocculation in a bacterium which showed better growth in Polypepton medium was investigated. The bacterium required a slight amount of calcium ion for the flocculation. The cell flocs were completely deflocculated not only by Actinase E, but also by EDTA. Deflocculation was also observed when the flocs were suspended in distilled water. In addition, the growth rate of the bacterium was not affected by the calcium ion in the culture medium.
社団法人日本生物工学会の論文
1986-02-25