Characterization of CitA-CitB Signal Transduction Activating Genes Involved in Anaerobic Citrate Catabolism in Escherichia coli

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概要

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• In Escherichia coli, CitA is a membrane-associated sensor histidine kinase that phosphorylates CitB, the response regulator. It is predicated to play a key role in anaerobic citrate catabolism. The citrate-binding site in CitA is located within its periplasmic domain, while the cytoplasmic domain (CitA-C) is involved in autophosphorylation. We found that autophosphorylation in vitro of CitA-C was induced by DTT. Using the whole set of CitA-C derivatives containing Cys-Ala substitution(s), Cys at 529 was found to be essential to the redox-sensing of autophosphorylation. The phosphorylated CitA-C transferred a phosphate to CitB. DNase-I footprinting assay indicated that CitB specifically bound on the intergenic region between the citA and citC genes. These results characterize the molecular mechanism of the CitA-CitB signal transduction system in E. coli.

• 社団法人 日本農芸化学会の論文
• 2009-02-23

著者

• Yamamoto K

  Kyushu Univ. Fukuoka

• YAMAMOTO Kaneyoshi

  Department of Agricultural Chemistry, Kinki University

• Oshima Taku

  Graduate School Of Information Sciences Nara Inst. Of Sci. And Technol.

• Ishihama A

  Division Of Molecular Biology Nippon Institute For Biological Science

• Ishihama Akira

  Department Of Biochemistry Institute For Virus Research Kyoto University

• Ishihama Akira

  Division Of Molecular Biology Nippon Institute For Biological Science

• Oshima Taku

  Graduate School Of Information Science Nara Institute Of Science And Technology

• Minagawa Shu

  Department Of Advanced Bioscience Kinki University

• Ogasawara Naotake

  Graduate School Of Information Sciences Nara Institute Of Science And Technology

• Ogasawara Naotake

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Ishihama Akira

  Dep. Of Frontier Bioscience And Res. Center For Micro-nano Technol. Hosei Univ.

• Fujita Nobuyuki

  Genome Analysis Center National Institute Of Technology And Evaluation

• MATSUMOTO Fumika

  Department of Advanced Bioscience, Kinki University

• Matsumoto Fumika

  Department Of Advanced Bioscience Kinki University

• Yamamoto Kaneyoshi

  Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University

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