Transcription of emrKY is Regulated by the EvgA-EvgS Two-Component System in Escherichia coli K-12

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概要

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• Spontaneous mutations have been isolated in Escherichia coli that result in the constitutive expression of an emrKY promoter. These mutations were found to be single-nucleotide substitutions within the linker region of the sensor protein EvgS, which is part of a two-component regulatory system along with EvgA. In the linker mutants (evgS1 and evgS4), emrKY expression became constitutive and MIC against sodium deoxycholate was 20mg/ml, eight-fold higher than in the wild type. Furthermore, the start site of transcription from the promoter of emrKY was identified; EvgA was showed to bind at the -52 to -84 region by the footprinting experiment.

• 社団法人日本農芸化学会の論文
• 2000-06-23

著者

• Yamamoto K

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• Yamamoto K

  Kyushu Univ. Fukuoka

• YAMAMOTO Kaneyoshi

  Department of Agricultural Chemistry, Kinki University

• Utsumi R

  Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University

• Utsumi Ryutaro

  Department Of Advanced Bioscience Kinki University

• Tanabe Hiroyuki

  Department Of Agricultural Chemistry Kinki University

• Tanabe H

  Kinki Univ. Nara Jpn

• KATO AKINORI

  Department of Surgery, Ibaraki Seinan Medical Center Hospital

• OHNISHI Hiroe

  Department of Agricultural Chemistry, Kinki University

• FURUTA Eiji

  Department of Agricultural Chemistry, Kinki University

• Furuta Eiji

  Department Of Agricultural Chemistry Kinki University

• Ohnishi Hiroe

  Department Of Agricultural Chemistry Kinki University

• Kato Akinori

  Department Of Agricultural Chemistry Kinki University

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