Characterization of Copper-Inducible Promoters Regulated by CpxA/CpxR in Escherichia coli
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概要
- 論文の詳細を見る
The copper stimulon in Escherichia coli consists of four regulons, the CueR-, CusS/CusR-, CpxA/CpxR-, and YedV/YedW regulons. E. coli mutants defective in cpxRA showed higher sensitivity to copper than the wild type. A total of 15 promoters were found to be induced in E. coli culture upon exposure to copper in a CpxA/CpxR-dependent manner. After gel-shift and DNase I foot-printing analyses, a conserved tandem repeat of pentanucleotide sequence, GTAAA(N)4–8GTAAA, with a conserved A of 4-bp upstream of each pentamer, was identified to be the CpxR-binding site. The difference in the orientation and location of the CpxR box is discussed with respect to the regulation mechanism among CpxR-regulon genes.
- 社団法人 日本農芸化学会の論文
- 2006-07-23
著者
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YAMAMOTO Kaneyoshi
Department of Agricultural Chemistry, Kinki University
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Ishihama Akira
Division Of Molecular Biology Nippon Institute For Biological Science
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Ishihama Akira
Dep. Of Frontier Bioscience And Res. Center For Micro-nano Technol. Hosei Univ.
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Yamamoto Kaneyoshi
Department of Frontier Bioscience and Research Center for Micro-Nano Technology, Hosei University
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