Effects of cya and cid Mutations on Cell Growth in Escherichia coli(Biological Chemistry)
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概要
- 論文の詳細を見る
A cya mutant (HY1025) in which cya expression was completely inhibited was constructed by use of the reversed genetic method. HY1025 was mutagenized by transposon Tn10. Next, cAMP-requiring mutants were isolated in minimal medium containing glucose. In the mutants, the locus mutagenized by Tn10 was different from cya and crp, and was tentatively named cid. The property requiring cAMP was dependent on both cya and cid. Furthermore, mutants (cid::Mud(Ap^r lac)) containing fusions of the lac gene to the cid gene were isolated. Among these strains, the expression of β-galactosidase was induced by cAMP. These experiments showed that Escherichia coli has cAMP-dependent growth regulation.
- 社団法人日本農芸化学会の論文
- 1987-02-23
著者
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Komano T
Kyoto Univ. Kyoto Jpn
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Komano Tohru
京都大学
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Kawamukai Makoto
Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, Univ
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Kawamukai Makoto
Department Of Natural Resources Faculty Of Agriculture Shimane University
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UTSUMI Ryutaro
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kinki University
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Utsumi R
Department Of Bioscience And Biotechnology Graduate School Of Agriculture Kinki University
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NODA Manjiro
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kinki University
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Komano T
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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