Purification and Mode of Action of Chitosanolytic Enzyme from Enterobacter sp. G-1
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概要
- 論文の詳細を見る
A chitosanolytic enzyme was purified from Enterobacter sp. G-1 by fractionation of 30% saturation with ammonium sulfate, isoelectric focusing, and Sephadex G-100 gel chromatography. The purified enzyme showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the molecular mass was estimated to be 50kDa. The enzyme degraded N-acetyl-chitooligosaccharides, glycol chitin, colloidal chitin, and colloidal chitosan (about 80% deacetylated), but did not degrade chitooligosaccharides, colloidal chitosan (100% deacetylated), or Micrococcus lysodeikticus cell walls. It hydrolyzed GlcNAc_<4-6> and colloidal chitin to GlcNAc_2, finally. The main cleavage site with GlcNAc_<3-6> was the second linkage from the non-reducing end, based on the pattern of pNp-GlcNAc_<2-5>. Colloidal chitosan was hydrolyzed to GlcNAc_2 and to similar partially N-acetylated chitooligosaccharides.
- 社団法人日本農芸化学会の論文
- 1993-03-23
著者
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KAWAMUKAI Makoto
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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MATSUDA Hideyuki
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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YAMASAKI Yukikazu
Institute of Industrial Science and Technology in Shimane Prefecture
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Oowatari Y
Shimane Univ. Matsue Jpn
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NAKAGAWA Tsuyoshi
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Matsuda H
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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YAMASAKI Yukikazu
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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OHTA Yukari
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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Hayashi Isami
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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Hayashi Isami
Applied Biological Sciences Faculty Of Agriculture Shimane University
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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