Glutamyl tRNA Synthetases and Glutamic Acid Induce Sexual Differentiation of Schizosaccharomyces pombe
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概要
- 論文の詳細を見る
The moc3 gene was screened out as an inducer of sexual differentiation in fission yeast Schizosaccharomyces pombe. We isolated a novel gene, named ers2, encoding mitochondrial glutamyl tRNA synthetase (mGluRS) as a Moc3 interacting element by the yeast two-hybrid system. Cytoplasmic glutamyl tRNA synthetase (cGluRS) also interacted with Moc3 in a yeast two-hybrid system. Disruption of ers1 (cGluRS) and of ers2 (mGluRS) indicated that these genes are both essential for the cell growth of S. pombe. We found that ers2 severely affected cell growth and decreased viability, but induced sexual differentiation of S. pombe when it was over-expressed. Over-expression of ers1 also stimulated sexual differentiation in S. pombe. These observations led us to test the effects of various amino acids on sexual differentiation. We found that glutamic acid, as well as other specific amino acids, such as tryptophan, methionine, and threonine, efficiently induced sexual differentiation in S. pombe. Our findings suggest a new regulatory mechanism where GluRSs and glutamic acid are involved in sexual differentiation in S. pombe.
- 社団法人 日本農芸化学会の論文
- 2009-06-23
著者
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Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Yakura Miyo
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Oowatari Yasuo
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Goldar Muniruzzaman
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Goldar Md.
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Paul Swapan
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Oowatari Yasuo
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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Kawamukai Makoto
Department Of Life Science And Biotechnology Faculty Of Life And Environmental Science Shimane Unive
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