Evidence That Escherichia coli ubiA Product Is a Functional Homolog of Yeast COQ2, and the Regulation of ubiA Gene Expression

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概要

• 論文の詳細を見る

• The Escherichia coli ubiA gene coding for 4-hydroxy benzoate octaprenyl transferase is thought to be a key enzyme of ubiquinone biosynthesis. Strains with ubiA disrupted were constructed by chromosomal gene replacement with the chloramphenicol resistance gene. The respiration-defective phenotype of the ubiA mutant was complemented by expression of the COQ2 gene encoding the 4-hydroxy benzoate hexaprenyl transferase of Saccharomyces cerevisiae and such strains produced ubiquinone-8. This strongly supports the idea that COQ2 catalyzes the same enzymatic reaction with UbiA and the substrate specificity of COQ2 is broad. Study of the expression of ubiA using an ubiA-lacZ fusion system showed that the ubiA expression was catabolite-repressed by glucose. This repression by glucose was obvious in the arcA mutant. ArcA is the positively acting transcriptional regulator of the oxygen regulated genes. The molecular mass of the protein product of ubiA was 32 kD, found using the over-expression of the ubiA gene.

• 社団法人日本農芸化学会の論文
• 1994-10-23

著者

• NAKAGAWA Tsuyoshi

  Research Institute of Molecular Genetics, Shimane University

• YUASA MIHOKO

  Department of Bioresource Science, Faculty of Agriculture Shimane University

• Suzuki K

  Kanazawa Univ. Kanazawa

• Ueda Minoru

  Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine

• Takahashi Nobutaka

  Frontier Research Program The Institute Of Physical And Chemical Research (rlken)

• Ueda M

  Keio Univ. Yokohama Jpn

• Yuasa Mihoko

  Marine Products Kimuraya Co. Ltd.

• Suzuki Kengo

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science

• Kawamukai Makoto

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Morita K

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Kawamukai M

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Matsuda H

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Matsuda Hideyuki

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Ueda Minoru

  Department Of Chemistry Faculty Of Science Tohoku University

• Ueda Minoru

  Department Of Applied Chemistry Aichi Institute Of Technology

• Nakagawa Tsuyoshi

  Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un

• Ueda Minoru

  Department 1 Product Development Division 1 Motorcycle R&d Center Honda R&d Co. Ltd.

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