Thermostabilization of Ovalbumin by an Alkaline Treatment : Examination for the Possible Implications of an Altered Serpin Loop Structure(Food & Nutrition Science)
スポンサーリンク
概要
- 論文の詳細を見る
Ovalbumin, a member of the serpin superfamily, is transformed via an intermediate state into a noncleaved, thermostabilized form (S-ovalbumin) during either the storage of unfertilized eggs or development of fertilized eggs ; essentially the same thermostabilization also occurs upon in vitro incubation of isolated ovalbumin under alkaline conditions. To investigate the implications of a partial insertion of the α-helical serpin loop into β-sheet A that has been proposed as a conformational mechanism for S-ovalbumin production, we examined the thermostabilization process of ovalbumin with different loop structures. When the thermostabilization processes were compared for the intact, P1-P1'-cleaved and P1-P1'/P8-P7-cleaved forms of egg white ovalbumin, both the rates for the conversion from the native to intermediate and from the intermediate to S-ovalbumin were almost indistinguishable among the three protein forms. Furthermore, the fully loop-inserted form of recombinant ovalbumin mutant R339T that had been thermostabilized by P1-P1' cleavage with Tm values from 72 to 88℃ was further thermostabilized by an alkaline treatment, yielding a final product (loop inserted S-ovalbumin) with a Tm value of 93℃. No significant difference was found between native ovalbumin and S-ovalbumin in respect of the rate of proteolytic cleavage of the loop by elastase and subtilisin. These data strongly suggest that S-ovalbumin is produced by a mechanism other than that of the partial loop insertion model.
- 社団法人日本農芸化学会の論文
- 2003-04-23
著者
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Hirose M
The Division Of Applied Life Sciences The Graduate School Of Agriculture Kyoto University
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Hirose Masaaki
Graduate School Of Agriculture Kyoto Univ.
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Takahashi Nobuyuki
Division of Cardiovascular Medicine, Department of Internal Medicine, Shimane University
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Hirose Masaaki
Division Of Applied Life Sciences The Graduate School Of Agriculture Kyoto University
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Hirose Masaaki
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Takahashi N
Univ. Tokyo Tokyo Jpn
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YAMAMOTO Hiroko
Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University
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YAMASAKI Masayuki
Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University
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ARII Yasuhiro
Division of Applied Life Sciences, The Graduate School of Agriculture, Kyoto University
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Takahashi Nobtaka
Department Of Agricultural Chemistry The University Of Tokyo:(presnet Addresses) The Institute Of Ph
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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Takahashi Nobuyuki
Division Of Cardiovascular Medicine Shimane University Faculty Of Medicine
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Tsuji Naoki
Department Of Clinical Laboratory Medicine Sapporo Medical University School Of Medicine
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Takeda Noboru
Faculty Of Pharmaceutical Sciences Josai University
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Arii Yasuhiro
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University:(present Address)s
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Maruyama Yukie
Laboratory Of Basic And Applied Molecular Biotechnology Graduate School Of Agriculture Kyoto Univers
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Takahashi N
Graduate School Of Agriculture Kyoto Univ.
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Yamasaki Masayuki
The Division Of Applied Life Sciences The Graduate School Of Agriculture Kyoto University
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Yamamoto Hiroko
Division Of Applied Life Sciences The Graduate School Of Agriculture Kyoto University
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Takahashi Nobuyuki
Division Of Cardiology Shimane University Faculty Of Medicine
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