Role of the Intrachain Disulfide Bond of Ovalbumin during Conversion into S-Ovalbumin
スポンサーリンク
概要
- 論文の詳細を見る
Disulfide-reduced and carboxymethylated ovalbumin was treated at pH 9.9 and 55℃ for 24h as a specific condition for preparation of S-ovalbumin. The stability and conformation of the product were investigated. Such alkaline treatment converted native protein to S-ovalbumin, but this modified ovalbumin was not stabilized, according to results of calorimetric analysis. Instead, it had lost its native like conformation; the magnitude of CD spectra decreased. The conformation after alkaline treatment was not clear, but the possibility of aggregation was excluded by electrophoretic analysis. These observations showed that the transformation of native ovalbumin into S-ovalbumin requires the presence of the disulfide bond.
- 社団法人日本農芸化学会の論文
- 1996-09-23
著者
-
Hirose M
The Division Of Applied Life Sciences The Graduate School Of Agriculture Kyoto University
-
Hirose M
Kyoto Univ. Kyoto Jpn
-
Hirose Masaaki
Graduate School Of Agriculture Kyoto Univ.
-
Hirose Masaaki
The Research Institute For Food Science Kyoto Univesity
-
Hirose Masaaki
Division Of Applied Life Sciences The Graduate School Of Agriculture Kyoto University
-
TATSUMI Eizo
The Research Institute for Food Science, Kyoto University
-
TAKAHASHI Nobuyuki
The Research Institute for Food Science, Kyoto University
-
ORITA Takuya
The Research Institute for Food Science, Kyoto University
-
Takahashi Nobtaka
Department Of Agricultural Chemistry The University Of Tokyo:(presnet Addresses) The Institute Of Ph
-
Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
-
Hirose Masaaki
The Research Institute For Food Science Kyoto University
-
Tsuji Naoki
Department Of Clinical Laboratory Medicine Sapporo Medical University School Of Medicine
-
Orita Takuya
The Research Institute For Food Science Kyoto University
-
Tatsumi E
Kyoto Univ. Kyoto Jpn
-
Tatsumi Eizo
The Research Institute For Food Science Kyoto University
-
Takahashi N
Graduate School Of Agriculture Kyoto Univ.
-
Takahashi Nobuyuki
The Research Institute For Food Science Kyoto University
関連論文
- NATURAL ANTIGENIC PEPTIDES OF GASTRIC SIGNET RING CELL CARCINOMAS
- ISOLATION OF BRASSINOSTEROID DEFICIENCY-INSENSITIVE MUTANTS OF ARABIDOPSIS
- DEVELOPMENT OF SELECTIVE AND POTENT BRASSINOSTEROID BIOSYNTHESIS INHIBITORS AND ISOLATION OF INHIBITOR INSENSITIVE MUTANTS OF ARABIDOPSIS
- Screnning of cytokinin biosynthesis inhibitors
- A novel albino mutant, alth deficient in chloroplast gene expression.
- Analysis of the Decaprenyl Diphosphate Synthase (dps) Gene in Fission Yeast Suggests a Role of Ubiquinone as an Antioxidant^1
- Cloning and Functional Expression of a Novel Geranylgeranyl Pyrophosphate Synthase Gene from Arabidopsis thaliana in Escherichia coli
- Production of Ubiquinone in Escherichia coli by Expression of Various Genes Responsible for Ubiquinone Biosynthesis
- Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
- Survivin as a predictor of cis-diamminedichloroplatinum sensitivity in gastric cancer patients
- Natural Antigenic Peptides from Squamous Cell Carcinoma Recognized by Autologous HLA-DR8-restricted CD4^+ T Cells
- Partial Characterization of a 17 kDa Acidic Protein, EFP, Induced by Thiocarbamate in the Early Flowering Phase in Asparagus Seedlings
- 41 Thiocarbamate-Induced Early Flowering Protein is Expressed in Male Asparagus Flower
- cDNA CLONING OF ENT-KAURENE SYNTHETASEB FROM THE IMMATURE SEEDS OF PUMPKIN
- Thermostabilization of Ovotransferrin by Anions for Pasteurization of Liquid Egg White
- Structural Characteristics of the Disulfide-reduced Ovotransferrin N-Lobe Analyzed by Protein Fragmentation
- Gelation of Porcine Plasma by Glutathione(Food & Nutrition)
- Microstructures of Bread Dough and the Effects of Shortening on Frozen Dough
- Gateway vectors for plant transformation
- The Cyanogenic Glucoside, Prunasin (D-Mandelonitrile-β-D-Glucoside), Is a Novel Inhibitor of DNA Polymerase β
- Refolding of Urea-Denatured Ovalbumin That Comprises Non-Native Disulfide Isomers
- Role of the Intrachain Disulfide Bond of Ovalbumin during Conversion into S-Ovalbumin
- Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal
- Anti-tumor Effect of Chemically Synthesized Sulfolipids Based on Sea Urchin's Natural Sulfonoquinovosylmonoacylglycerols
- Mammalian Spermidine Synthase : Identification of Cysteine Residues and Investigation of the Putrescine Binding Site(Analytical Biochemistry)
- Synthetic Decarboxylated S-Adenosyl-L-methionine as a Substrate for Aminopropyl Transferases
- Periplasmic Secretion of Functional Ovotransferrin N-Lobe in Escherichia coli
- Inhibition of Prostaglandin Biosynthesis by 3-Nitro-2,4,6-trihydroxybenzamides
- Inhibition of Human 5-Lipoxygenase by 3-Nitro-2,4,6-trihydroxybenzamide Derivatives(Biological Chemistry)
- Structure-Activity Relationship of 3-Nitro-2,4,6-trihydroxybenzamide Derivatives in Photosynthetic Electron Transport Inhibition(Pesticide Chemistry)
- Syntheses of 3-Nitro-2,4,6-trihydroxy-benzamide and Thiobenzamide Derivatives as Highly Potent Inhibitors of Photosynthetic Electron Transport(Pesticide Chemistry)
- Inhibition of Photosynthesis by 3-Acyl-2,4,6-trihydroxythiobenzamide Derivatives(Organic Chemistry)
- Photosynthetic Electron Transport Inhibition by 3-Acyl-2, 4, 6-trihydroxybenzamide Derivatives(Organic Chemistry)
- Gibberellin Biosynthesis in Bambusoideae
- Effect of Ethylene and Gibberellin on the Elongation of Rice Seedlings(Oryza sativa L. )
- Effects of Low Irradiance Stress on Gibberellin Levels in Pea Seedlings : GROWTH AND DEVELOPMENT
- Effect of Ethylene and Gibberellin on theElongation of Rice Seedlings (Oryza sativa L.)
- Thermostability of Refolded Ovalbumin and S-Ovalbumin
- Thermostabilization of Ovalbumin by an Alkaline Treatment : Examination for the Possible Implications of an Altered Serpin Loop Structure(Food & Nutrition Science)
- Periplasmic Secretion of Native Ovalbumin without Signal Cleavage in Escherichia coli(Biochemistry & Molecular Biology)
- Structural Properties of Recombinant Ovalbumin and Its Transformation into a Thermostabilized Form by Alkaline Treatment
- Thiol-dependent Gelation of Conalbumin in the Presence of Denaturant(Food & Nutrition)
- Thiol-dependent Gelation of Conalbumin: Use of Thiosalicylic Acid and Its Removal from the Gel(Food & Nutrition)
- Effects of Anions on the Thiol-dependent Gelation of Conalbumin(Food & Nutrition)
- Conformation Changes and Subsequent Gelation of Conalbumin by a Thiol Reagent(Biological Chemistry)
- Characterization of Endogenous Gibberellins in Dwarf Rice Mutants
- Cloning and Sequence Analysis of Genes for Cyclophilin from Arabidopsis thaliana : GENE STRUCTURE AND EXPRESSION
- Effects of Fluorogibberellins on Plant Growth and Gibberellin 3β-Hydroxylases
- Effects of Ethylene and Gibberellins on the Elongation of Rice Seedlings (Oryza sativa L.)
- Inhibition of ent-Kaurene Synthase by Quaternary Ammonium Growth Retardants
- Effects of Gibberellins and Prohexadione on the Activities of Oryzain and α-Amylase in Rice Seeds : PROTEINS, ENZYMES AND METABOLISM
- 12 Activities of natural gibberellins and related compounds in inducing gene expressions of α-amylase and oryzain in rice aleurone tissues
- Effects of 3-Methylgibberellin Analogs on Gibberellin 3β-Hydroxylases and Plant Growth
- Cytokinin inducible genes in culture green tobacco cells.
- Analysis of phyotohormone contents in tobacco CND41 antisense transformants.
- Analyzing the function of a 41 kD chloroplast-nucleoid DNA-binding protein (CND41) by F2 progeny of antisense-transgenic tobacco
- A CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN AND ITS FUNCTION IN CHLOROPLAST GENE EXPRESSION
- Purification, Characterization and Gene Analysis of N-Acetylglucosaminidase from Enterobacter sp. G-1
- Molecular Cloning, Nucleotide Sequencing, and Regulation of the chiA Gene Encoding One of Chitinases from Enterobacter sp. G-1
- Purification and Characterization of the Chitinase (ChiA) from Enterobacter sp. G-1
- Structure of Malformin B, a Phytotoxic Metabolite Produced by Aspergillus niger
- Structure of Malformin A, a Phytotoxic Metabolite Produced by Aspergillus niger
- ANALYSIS OF INTERACTION OF FLUORESCENCE-LABELED BIOACTIVE GIBBERELLIN OR ABSCISIC ACID WITH BARLEY ALEURONE PROTOPLASTS
- Control by Light of Hypocotyl Elongation and Levels of Endogenous Gibberellins in Seedlings of Lactuca sativa L. : GROWTH AND DEVELOPMENT
- Identification of Brassinosteroids with Epimerized Substituents and/or the 23-Oxo Group in Pollen and Anthers of Japanese Cedar
- Cloning and Sequencing Analysis of Alginate Lyase Genes from the Marine Bacterium Vibrio sp. O2
- Effects of Fucoidan from Mozuku on Human Stomach Cell Lines
- Genetic Analysis of chs1^+ and chs2^+ Encoding Chitin Synthases from Schizosaccharomyces pombe
- Semi Quantification of Gibberellins in the Anthers of Thermosensitive Genetic Male Sterile Rice (Oryza sativa L. cv. PL12)
- Downregulation of Tie2 gene by a novel antitumor sulfolipid, 3'-sulfoquinovosyl-1'-monoacylglycerol, targeting angiogenesis
- Olfactomedin 4 promotes S-phase transition in proliferation of pancreatic cancer cells
- Specific overexpression of OLFM4^ mRNA in colon, breast and lung cancer tissues detected using quantitative analysis
- Arabidopsis obtusifoliol 14α-demethylase (CYP51) in sterol biosynthetic pathway
- POSSIBLE ROLE OF CND41 IN DEGRADATION OF RUBISCO
- Accumulation of CND41 and expression of chloroplastic genes in cultured tobacco cell
- Protease activity of CND41, a chloroplast DNA-binding protein
- Limited Proteolysis of Disulfide-reduced Ovalbumin by Subtilisin
- Qualitative and Quantitative Analysis of Endogenous Gibberellins in Raphanus sativus L. during Cold Treatment and the Subsequent Growth
- Distribution of Gibberellins in Bamboo Shoots
- Endogenous Gibberellins from Young Shoots of Three Bamboo Species
- Conformational State of Disulfide-Reduced Ovalbumin at Acidic pH
- Involvement of Ovotransferrin in the Thermally Induced Gelation of Egg White at around 65℃
- Thiol-dependent Gelation of the Domain I-truncated Fragment of Bovine Serum Albumin
- Highly Ordered Molten Globule-Like State of Ovalbumin at Acidic pH: Native-Like Fragmentation by Protease and Selective Modification of Cys367 with Dithiodipyridine
- The Structural Mechanism for Iron Uptake and Release by Transferrins
- Roles of the Domain I Segment in Emulsifying Properties of Bovine Serum Albumin
- Partial Purification of a Doluble Hibberellin-Binding Protein from Mung Bean Hypocotyls
- Inhibitory Effect of Monoacylglycerol on Fatty Acid Uptake into Rat Intestinal Epithelial Cells
- Biochemical and Physiological Characteristics of Fat Cell
- Oleyl Alcohol Inhibits Intestinal Long-Chain Fatty Acid Absorption in Rats
- 4-O-Caffeoylshikimic and 4-O-(p-Coumaroyl)shikimic Acids from the Dwarf Tree Fern, Dicksonia antarctica
- Bioactive Gibberellin and Its Metabolism in Shoots of Phaseolus vulgaris
- A Role for Survivin in Radioresistance of Pancreatic Cancer Cells
- Gene Expression for Suppressors of Telomerase Activity (Telomeric-repeat Binding Factors) in Breast Cancer
- Anti-GA_3-Me Antiserum with High Specificity toward the 13-Hydroxyl Group of C_-Gibberellins
- Structural and Functional Characterization of Recombinant Human Serum Transferrin Secreted from Pichia pastoris
- Development of a Novel PPARγ Ligand Screening System Using Pinpoint Fluorescence-Probed Protein
- Endogenous Gibberellins in the Immature Seed and Pericarp of Loquat (Eriobotrya japonica)
- A Method for the Simultaneous Determination of 3T3-L1 Adipocyte Metabolites by Liquid Chromatography/Mass Spectrometry Using [13C]-stable Isotopes
- Soymorphin-5, a soy-derived μ opioid peptide, improves glucose and lipid metabolism in diabetic KK-Ay mice