Production of Ubiquinone in Escherichia coli by Expression of Various Genes Responsible for Ubiquinone Biosynthesis

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概要

• 論文の詳細を見る

• Ubiquinone(-10), known as a component of the electron transfer system in many organisms, has been used for the treatment of heat disease. No attempt at developing an approach for overproduction of ubiquinone by genetic engineering has been reported, presumably because of the limited number of genes involved in ubiquinone biosynthesis have been cloned.In the present study we oberproduced ubiquinone in Escherichia coli using all available genes involved in ubiquinone biosynthesis. Two genes were found to be important for the production of ubiquinone, ubiA, which encodes p-hydroxybenzoate-polyprenyl pyrophosphata transferase and ispB, which encodes polyprenyl pyrophosphate synthetase. We succeeded in achieving a level of ubiquinone priduction three times that of the wild-type cells by genetic engineering.

• 社団法人日本生物工学会の論文
• 1995-05-25

著者

• Suzuki Kengo

  Department of Cardiology, St. Marianna University School of Medicine

• Okada K

  Tokyo Univ. Pharmacy And Life Sci. Hachioji Jpn

• OKADA Kazunori

  Department of Biology, Tokyo Gakugei University

• NAKAGAWA Tsuyoshi

  Research Institute of Molecular Genetics, Shimane University

• KAWAMUKAI Makoto

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science

• MATSUDA Hideyuki

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science

• Zhu XuFen

  Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima

• YUASA MIHOKO

  Department of Bioresource Science, Faculty of Agriculture Shimane University

• Okada K

  Communications Research Laboratory Ministry Of Posts And Telecommunications

• Okada K

  The Design Technology Development Laboratory Ic Group Sharp Corporation

• Suzuki K

  Kanazawa Univ. Kanazawa

• Takahashi Nobutaka

  Frontier Research Program The Institute Of Physical And Chemical Research (rlken)

• Zhu X

  Xiamen Univ. Xiamen Chn

• Okada Kimiharu

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science

• Yuasa Mihoko

  Marine Products Kimuraya Co. Ltd.

• Suzuki Kengo

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science

• Kawamukai Makoto

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Zhu Xufen

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science

• Morita K

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Kawamukai M

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Takahashi Nobuyuki

  Hokkaido University Of Education

• Matsuda H

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Matsuda Hideyuki

  Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane

• Nakagawa Tsuyoshi

  Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un

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