Effects of the Escherichia coli sfsA Gene on mal Genes Expression and a DNA Binding Activity of SfsA
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概要
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The sfsA gene was identified as one of the sfs genes the over-expression of which stimulates maltose fermentation of the Mal^- Escherichia coli strain MK2001 (crp^*1,cya : Km^r). Expression from the malPQ promoter, which was measured using a chromosomally integrated malPp-lacZ fusion, was induced by over-expressing the sfsA gene in the crp^*1,cya : Km^r strain. The level of the MalE protein was increased in crp^*1,cya : Km^r cells over-producing SfsA. The SfsA protein was purified to homogeneity and tested for DNA binding activity. The purified SfsA protein binds to DNA non-specifically. All these results may suggest that SfsA functions as a DNA binding protein to induce the mal genes in coordination with CRP^*1.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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KAWAMUKAI Makoto
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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Akimoto-tomiyama Chiharu
National Inst. Of Agrobiological Sciences
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Kawamukai Makoto
Department Of Life Science And Biotechnology Shimane University
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Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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TAKEDA Kazuhiko
Department of Life Science and Biotechnology, Shimane University
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AKIMOTO Chiyuri
Department of Life Science and Biotechnology, Shimane University
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Akimoto Chiyuri
Department Of Life Science And Biotechnology Shimane University
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Akimoto Chiharu
Department Biochemistry National Institute Of Agrobiological Science
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Takeda Kazuhiko
Department Of Life Science And Biotechnology Shimane University
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Takeda Kazuhiko
Department Of Environmental Dynamics And Management Graduate School Of Biosphere Science Hiroshima U
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Kawamura Makoto
Department Of Gynecology And Obstetrics Kyoto University Graduate School Of Medicine
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Akimoto Chiyuri
Department Biochemistry, National Institute of Agrobiological Science
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