Simple and Effective Gap-Repair Cloning Using Short Tracts of Flanking Homology in Fission Yeast
スポンサーリンク
概要
- 論文の詳細を見る
Gap-repair cloning for plasmid construction in budding yeast is very effective and often used. In contrast, the same method is not widely used in fission yeast, because of a shortage of information on it. Here we describe simple and effective gap-repair cloning for plasmid construction using short tracts of flanking homology. By this method, we combined concentrated DNA fragments with short (20 bp) tracts of flanking homology with the marker gene or the pre-existing gene module. In addition, we found that this method can be applied to one-step cloning of multiple DNA fragments to construct a fusion gene.
- 社団法人 日本農芸化学会の論文
- 2010-03-23
著者
-
Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
-
Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
-
MATSUO Yuzy
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
-
KISHIMOTO Hayafumi
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
-
HORIUCHI Tomitaka
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
-
TANAE Katsuhiro
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
-
Matsuo Yuzy
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
-
Kishimoto Hayafumi
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
-
Horiuchi Tomitaka
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
-
Tanae Katsuhiro
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
関連論文
- Analysis of the Decaprenyl Diphosphate Synthase (dps) Gene in Fission Yeast Suggests a Role of Ubiquinone as an Antioxidant^1
- Cloning and Functional Expression of a Novel Geranylgeranyl Pyrophosphate Synthase Gene from Arabidopsis thaliana in Escherichia coli
- Production of Ubiquinone in Escherichia coli by Expression of Various Genes Responsible for Ubiquinone Biosynthesis
- Identification and Subcellular Localization of Two Solanesyl Diphosphate Synthases from Arabidopsis thaliana
- Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal
- Purification, Characterization and Gene Analysis of N-Acetylglucosaminidase from Enterobacter sp. G-1
- Purification and Characterization of the Chitinase (ChiA) from Enterobacter sp. G-1
- Cloning and Sequencing Analysis of Alginate Lyase Genes from the Marine Bacterium Vibrio sp. O2
- Effects of Fucoidan from Mozuku on Human Stomach Cell Lines
- Genetic Analysis of chs1^+ and chs2^+ Encoding Chitin Synthases from Schizosaccharomyces pombe
- Interaction between a Negative Regulator (Msa2/Nrd1) and a Positive Regulator (Cpc2) of Sexual Differentiation in Schizosaccharomyces pombe
- Truncated Sla1 Induces Haploid Meiosis through the Path1-Mei2 System in Fission Yeast
- Involvement of Moc1 in Sexual Development and Survival of Schizosaccharomyces pombe
- Effects of the Escherichia coli sfsA Gene on mal Genes Expression and a DNA Binding Activity of SfsA
- Functional Conservation between Fission Yeast moc1/sds23 and Its Two Orthologs, Budding Yeast SDS23 and SDS24, and Phenotypic Differences in Their Disruptants
- Mutational Analysis of the fic Promoter Recognized by RpoS (σ^) in Escherichia coli
- Two Glutamic Acids in Chitosanase A from Matsuebacter chitosanotabidus 3001 Are the Catalytically Important Residues
- Identification of a 14-3-3 Protein from Lentinus edodes That Interacts with CAP (Adenylyl Cyclase-associated Protein), and Conservation of This Interaction in Fission Yeast
- Two Cytosolic Cyclophilin Genes of Arabidopsis thaliana Differently Regulated in Temporal-and Organ-Specific Expression
- Evidence That Escherichia coli ubiA Product Is a Functional Homolog of Yeast COQ2, and the Regulation of ubiA Gene Expression
- Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
- Genetic Analysis of the sam Mutations, Which Induce Sexual Development with No Requirement for Nutritional Starvation in Fission Yeast
- Identification of sam4 as a rad24 Allele in Schizosaccharomyces pombe
- Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
- Development of a Series of Gateway Binary Vectors Possessing a Tunicamycin Resistance Gene as a Marker for the Transformation of Arabidopsis thaliana
- Two Sec13p Homologs, AtSec13A and AtSec13B, Redundantly Contribute to the Formation of COPII Transport Vesicles in Arabidopsis thaliana
- Glutamyl tRNA Synthetases and Glutamic Acid Induce Sexual Differentiation of Schizosaccharomyces pombe
- Complex Formation, Phosphorylation, and Localization of Protein Kinase A of Schizosaccharomyces pombe upon Glucose Starvation
- Simple and Effective Gap-Repair Cloning Using Short Tracts of Flanking Homology in Fission Yeast
- Functional Expression of Chitinase and Chitosanase, and Their Effects on Morphologies in the Yeast Schizosaccharomyces pombe(Biochemistry & Molecular Biology)
- Directed Evolution to Enhance Secretion Efficiency and Thermostability of Chitosanase from Mitsuaria chitosanitabida 3001
- Complex Formation, Phosphorylation, and Localization of Protein Kinase A of Schizosaccharomyces pombe upon Glucose Starvation
- Two Sec13p Homologs, AtSec13A and AtSec13B, Redundantly Contribute to the Formation of COPII Transport Vesicles in Arabidopsis thaliana
- Expression Analysis of Arabidopsis thaliana Small Secreted Protein Genes
- Expression Analysis of Arabidopsis thaliana Small Secreted Protein Genes