Development of a Series of Gateway Binary Vectors Possessing a Tunicamycin Resistance Gene as a Marker for the Transformation of Arabidopsis thaliana
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概要
- 論文の詳細を見る
We made two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing a UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene driven by the nopaline synthase promoter (Pnos) as a tunicamycin resistance marker for the transformation of Arabidopsis thaliana. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP. Selection of transformants was successful on plates containing 0.15 mg/L of tunicamycin. These vectors were compatible with existing pGWB and R4pGWB vectors for kanamycin, hygromycin B, and BASTA® selection, and are useful new tools for making transgenic Arabidopsis.
- 2011-04-23
著者
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Tanaka Yuji
Department Of Electronics Faculty Of Engineering Nagoya University
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Takahashi N
Plant Functions Laboratory Riken
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Koizumi Nozomu
Graduate School Of Agricultural Sciences Osaka Prefectural University
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Nakano Takeshi
Riken Advanced Sci. Inst.
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
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NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
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Kawamukai Makoto
Department Of Natural Resources Faculty Of Agriculture Shimane University
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Kawamukai Makoto
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Nakamura Shinya
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Takahashi Nobuyuki
Hokkaido University Of Education
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Tanaka Yuji
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Nakano Takeshi
Plant Functions Lab. The Inst. Of Physical And Chemical Res.
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Nakano Takeshi
Plant Functions Lab.
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Nakamura Shinya
Department Of Clinical Pharmaceutical Science Faculty Of Pharmaceutical Sciences Okayama University
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Nakamura Shinya
Department Of Chemistry University Of Tsukuba
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Tanaka Yuji
Department Of Cell Therapy And Transplantation University Of Tokyo
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Tanaka Yuji
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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Takahashi Nobuyuki
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
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Takahashi Noriko
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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NAKAGAWA Tsuyoshi
Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology
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