Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
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概要
- 論文の詳細を見る
We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter:reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoter-attB1-reporter construct. The reporters employed in R4L1pGWBs were β-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).
- 社団法人 日本農芸化学会の論文
著者
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Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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NAKAGAWA Tsuyoshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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ISHIGURO Sumie
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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KAWAMUKAI Makoto
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
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Kimura Tetsuya
Department Of Sustainable Resource Science Graduate School Of Of Bioresources Mie University
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Nakao Akihide
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
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