Partial Purification of a Doluble Hibberellin-Binding Protein from Mung Bean Hypocotyls
スポンサーリンク
概要
- 論文の詳細を見る
A soluble binding protein specific for GA_4, GA_7 and GA_9 was partially purified from mung bean hypocotyls, and its characteristics were examined. Affinity chromatography using immobilized GA_3 coupled to Sepharose 4B via the C-7 carboxyl group was very effective for purification of the protein. The molecular weight of the protein in its native state was estimated to be 150-200 kDa by gel-permeation chromatography. This protein may be a heterooligomer consisting of two subunits (23 kDa and 35 kDa). The optimum pH for binding of GA_4 to the protein was around 6.0 and the apparent dissociation constant (K_d) was 3×10^<-7> M.
- 日本植物生理学会の論文
著者
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MUROFUSHI Noboru
Faculty of Bioresource Sciences, Akita Prefectural University
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YAMAGUCHI Isomaro
Department of Applied Biological Chemistry, The University of Tokyo
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NAKAJIMA Masatoshi
Department of Applied Biological Sciences, The University of Tokyo
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MUROFUSHI Noboru
Department of Biotechnology, Akita Prefectural University
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Maejima Noboru
Department Of Biotechnology Akita Prefectural University
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Nonaka Masahiko
Food Research & Development Laboratories Ajinomoto Co. Inc.
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KANAZAWA Kenji
Department of General Internal Medicine, Hyogo Prefecture Health Promotion Association
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Kizawa Satoru
Department of Agricultural Chemistry, The University of Tokyo
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Yamaguchi Isomaro
Department Of Applied Biological Chemistry The University Of Tokyo
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Takahashi Nobtaka
Department Of Agricultural Chemistry The University Of Tokyo:(presnet Addresses) The Institute Of Ph
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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Murofushi Noboru
Department Of Agricultural Chemistry The University Of Tokyo
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Yamaguchi Isomaro
Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo
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Nakajima Masatoshi
Department Of Applied Biological Chemistry The University Of Tokyo
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Kaihara Sumiko
Lab.Expt.Biol., kyoyama-Hatsunecho
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Kaihara Sumiko
Lab.expt.biol. Kyoyama-hatsunecho
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Yamaguchi I
Department Of Applied Biological Chemistry University Of Tokyo
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Kaihara S
Lab.expt.biol. Kyoyama-hatsunecho
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Nakajima Masatoshi
Department Of Applied Biological Chemistry University Of Tokyo
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Nakayama M
Dept. Biol. Sci. Fac. Biosci. Biotechnol. Tokyo Inst. Technol.
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SAKAI Shingo
Institute of Biological Science, University of Tsukuba
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Tsuji Naoki
Department Of Clinical Laboratory Medicine Sapporo Medical University School Of Medicine
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Nonaka M
Kyushu Univ. Fukuoka Jpn
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Kizawa Satoru
Department Of Agricultural Chemistry The University Of Tokyo
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Takahashi N
Graduate School Of Agriculture Kyoto Univ.
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Kanazawa Kenji
Department Of Agricultural Chemistry The University Of Tokyo
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TAKAHASHI Nobutaka
RIKEN
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Sakai S
Kyushu Univ.
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Kaihara Sumiko
Department Of Agricultural Chemistry The University Of Tokyo
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Nonaka M
Department Of Applied Biological Chemistry The University Of Tokyo
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Sakai Shingo
Institute For Biochemical Regulation Faculty Of Agriculture Nagoya University
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