Distribution of Gibberellins and Expressional Analysis of GA 20-oxidase Genes of Morning Glory during Fruit Maturation
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概要
- 論文の詳細を見る
Gibberellins A1⁄3 (GA1⁄3) and GA20 appeared earlier in surrounding tissues (pericarps/carpel/placenta) than in developing seeds of morning glory. The content of GA1⁄3 became higher in seeds than in the surrounding tissues at 9 days after anthesis (DAA), while that of GA20 stayed lower in seeds even at 12 DAA, suggesting the possibility that GA20 was translocated into seeds from the surrounding tissues and converted to GA1⁄3. The site of biosynthesis of GA20 in the fruits was determined by RNA-blotting and in situ hybridization of GA 20-oxidase genes (InGA20ox1, InGA20ox2). InGA20ox1 was not expressed in the surrounding tissues but in seeds, while no signal due to InGA20ox2 was detected in neither tissue. The expression of InGA20ox1 started in the seed coat near the hilum and spread in the seed coat like those of GA 3-oxidase and GA-inducible α-amylase genes. These observations suggest that GA biosynthesis is tissue-specifically and time-dependently regulated in the fruit of morning glory.
- 社団法人 日本農芸化学会の論文
- 2005-02-23
著者
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NAKAYAMA Akira
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokka
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YAMAGUCHI Isomaro
Department of Applied Biological Chemistry, The University of Tokyo
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NAKAJIMA Masatoshi
Department of Applied Biological Sciences, The University of Tokyo
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Yamaguchi Isomaro
Department Of Applied Biological Chemistry The University Of Tokyo
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Yamaguchi Isomaro
Department Of Applied Biological Chemistry University Of Tokyo
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Yamaguchi Isomaro
Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo
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Nakajima Masatoshi
Department Of Applied Biological Chemistry The University Of Tokyo
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Nakayama A
Fukushima Univ.
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Nakayama Akira
Department Of Internal Medicine Fukui Cardiovascular Center
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Yamaguchi I
Department Of Applied Biological Chemistry University Of Tokyo
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Nakajima Masatoshi
Department Of Applied Biological Chemistry University Of Tokyo
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Nakayama M
Dept. Biol. Sci. Fac. Biosci. Biotechnol. Tokyo Inst. Technol.
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Yamaguchi I
The Institute Of Physical And Chemical Research (riken)
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Nonaka M
Department Of Applied Biological Chemistry The University Of Tokyo
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Nakayama Akira
Department Of Adoministration And Social Sciences Fukushima University
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Yamaguchi Isomaro
Dept. Appl. Biol. Chem. Tokyo Univ.
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