Radioimmunoassay of Gibberellins A_5 and A_20

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概要

• 論文の詳細を見る

• Polyclonal anti-GA_5-antiserum and anti-GA_<20>-antiserum were prepared by immunizing rabbits with conjugates of N-GA_5-β-alanyl BSA and N-GA_<20>-β-alanyl BSA. By radioimmunoassay using these antisera, GA_5 and GA_<20> in developing fruits of Pharbitis nil Chois were analyzed after several purification steps and the results were compared with those obtained by GC-MS to examine the reliability of the analysis by radioimmunoassay. The analytical results by radioimmunoassay did not agree with those by GC-MS until two-step purifications by HPLC, indicating that samples must be suitably purified prior to radioimmunoassay to obtain reliable results.

• 日本植物生理学会の論文

著者

• TAKAHASHI Nobutaka

  Department of Biochemistry University of Shizuoka School of Pharmaceutical Science

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• MUROFUSHI Noboru

  Department of Biotechnology, Akita Prefectural University

• NAKAGAWA Ryusuke

  Department of Cell Regulation, Faculty of Medicine, Kagawa University

• Takahashi Nobutaka

  Department Of Agricultural Chemistry The University Of Takyo

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Murofushi Noboru

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Niwa Rimpei

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Weiler E

  Ruhr‐univ. Bochum Deu

• Kurogochi Shin

  Nihon Tokushu-Noyaku Co. Ltd.

• Weiler Elmar

  Pflanzenphysiologie, niversitaet Osnobrueck

• Nakagawa Ryusuke

  Department Of Cell Regulation Faculty Of Medicine Kagawa University

• Takahashi Nobutaka

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

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