Isolation of Gibberellin-binding Proteins by Aflinity Chromatography
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概要
- 論文の詳細を見る
Gibberellin-binding proteins (GBPs) were isolated from a crude extract of etiolated maize seedlings by affinity chromatography on gibberellin A_3(GA_3)-linked enhance-aminohexyl(EAH)-Sepharose 4B. The isolated proteins had a binding activity for [^3H]-GA_4. The binding site with dissociation constant of 0. 5μM for GA_4 was detected in the proteins by the binding assay with the procedures of ammonium sulfate precipitation. The binding of [^3H]-GA_4 to GBPs was reversible and could be inhibited by the addition of GA_1 and GA_3. An inactive gibberellin, GA_<13>, had no inhibitory effect on the binding of [^3H]-GA_4 to GBPs.
- 社団法人日本農芸化学会の論文
- 1994-05-23
著者
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YAMAGUCHI Isomaro
Department of Applied Biological Chemistry, The University of Tokyo
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NAKAJIMA Masatoshi
Department of Applied Biological Sciences, The University of Tokyo
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Nonaka Masahiko
Food Research & Development Laboratories Ajinomoto Co. Inc.
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Yamaguchi Isamu
Laboratory For Remediation Research Plant Science Center Riken
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Yamaguchi Isomaro
Department Of Applied Biological Chemistry The University Of Tokyo
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Yamaguchi Isomaro
Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo
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Nakajima Masatoshi
Department Of Applied Biological Chemistry The University Of Tokyo
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Yamaguchi I
Department Of Applied Biological Chemistry University Of Tokyo
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Nakajima Masatoshi
Department Of Applied Biological Chemistry University Of Tokyo
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Nakayama M
Dept. Biol. Sci. Fac. Biosci. Biotechnol. Tokyo Inst. Technol.
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SAKAI Shingo
Institute of Biological Science, University of Tsukuba
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Nonaka M
Kyushu Univ. Fukuoka Jpn
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Sakai S
Kyushu Univ.
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Nonaka M
Department Of Applied Biological Chemistry The University Of Tokyo
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Sakai Shingo
Institute For Biochemical Regulation Faculty Of Agriculture Nagoya University
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