Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Multiple-gene transformation is required to improve or change plant metabolisms effectively ; but this manystep procedure is time-consuming and costing. We succeeded in the metabolic engineering of tobacco plants by introducing multiple genes as a bacteria-type operon into a plastid genome. The tobacco plastid was transformed with a polycistron consisting of three bacterial genes for the biosynthesis of a biodegradable polyester, polyhydroxybutyrate (PHB). Accumulation of PHB in the leaves of the transgenic tobacco indicated that the introduced genes were polycistronically expressed. This "phyto-fermentation" system can be used in plant production of various chemical commodities and pharmaceuticals.
- 社団法人日本農芸化学会の論文
- 2001-07-23
著者
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Shikanai Toshiharu
Graduate school of Science and Technology, Nara Institute of Science and Technology
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YAMAGUCHI Isamu
Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN)
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Shikanai Toshiharu
Department of Botany, Graduate School of Science, Kyoto University
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ARAI Yuko
Microbial Toxicology Laboratory, RIKEN Institute
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NAKASHITA Hideo
Microbial Toxicology Laboratory, RIKEN Institute
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DOI Yoshiharu
RIKEN Institute
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Nakashita Hideo
Environmental Molecular Biology Laboratory Discovery Research Institute Riken
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DOI Yoshiharu
Microbial Toxicology Laboratory, RIKEN Institute
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Yamaguchi I
Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie
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Yamaguchi Isomaro
Department Of Applied Biological Chemistry The University Of Tokyo
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Yamaguchi Isamu
Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie
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Shikanai Toshiharu
Graduate School Of Agriculture Kyushu University
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Shikanai Toshiharu
Riken Institute
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Shikanai Toshiharu
Nichirei Corporation
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Yamaguchi I
Department Of Applied Biological Chemistry University Of Tokyo
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Arai Y
Microbial Toxicology Laboratory Riken Institute:graduate School Of Science And Engineering Saitama U
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Doi Yoshiharu
Polymer Chemistry Laboratory Riken Institute
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Yamaguchi I
The Institute Of Physical And Chemical Research (riken)
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Yamaguchi Isomaro
Dept. Appl. Biol. Chem. Tokyo Univ.
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YAMAGUCHI Isamu
Microbial Toxicology Laboratory and Biodesign Research Group, RIKEN(The Institute of Physical and Chemical Research)
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NAKASHITA Hideo
Microbial Toxicology Laboratory, RIKEN
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