cDNA Subtractive Cloning of Genes Expressed during Early Stage of Appressorium Formation by Magnaporthe grisea
スポンサーリンク
概要
- 論文の詳細を見る
The conidial germ tube of the fungus Magnaporthe grisea differentiates an infection-specific structure, an appressorium, for penetration into the host plant. Formation of the appressorium is also observed on synthetic solid substrata such as polycarbonate. We found that a plant lectin, concanavalin A, specifically suppressed the appressorium formation without affecting the germling adhesion if it was applied within 2-3 hours after germination. Standing on the result, we constructed a cDNA library that represents the early stage of germ tube development and/or appressorium formation from the 2.5-hour-old germ tubes using a cDNA subtraction strategy by the combination of the biotin labeled driver method and adapter primed PCR method. Out of 686 colonies of the library, 158 distinct clones' nucleotide sequences were partially analyzed. Some clones' expression patterns were detected by RT-PCR and from those results, our library seemed to well represent the objective developmental stage of M.grisea.
- 社団法人日本農芸化学会の論文
- 1999-08-23
著者
-
Xiao Jin-zhong
Food Science and Technology Institute, Morinaga Milk Industry Co., Ltd.
-
Xiao J‐z
Morinaga Milk Ind. Co. Ltd. Zama Jpn
-
KAMAKURA Takashi
Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN)
-
XIAO Jin-zhong
Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN)
-
CHOI Woo-Boon
Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN)
-
KOCHI Takamasa
Tokyo University of Agriculture and Technology
-
YAMAGUCHI Syuichi
Tokyo University of Agriculture and Technology
-
TERAOKA Tohru
Tokyo University of Agriculture and Technology
-
YAMAGUCHI Isamu
Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN)
-
Choi Woo-bong
Microbial Toxicology Laboratory The Institute Of Physical And Chemical Research (riken):(present Add
-
Kamakura T
Graduate School Of Agriculture Tokyo University Of Agriculture And Technology
-
Kamakura Takashi
Riken The Institute Of Physical And Chemical Research
-
Kamakura Takashi
Microbial Toxicology Laboratory The Institute Of Physical And Chemical Research (riken)
-
Teraoka T
Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology (tuat)
-
Teraoka T
Tokyo Univ. Agriculture & Technol. Tokyo Jpn
-
Yamaguchi I
Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie
-
Yamaguchi Isamu
Laboratory For Remediation Research Plant Science Center Riken
-
Yamaguchi I
Riken The Institute Of Physical And Chemical Research
-
Teraoka Tohru
Graduate School Of Agriculture Tokyo University Of Agriculture And Technology
-
Yamaguchi Isamu
Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie
-
Yamaguchi I
Riken (the Institute Of Physical And Chemical Research) Plant Science Center
-
Togashi Kana
Graduate School Of Agriculture Tokyo University Of Agriculture And Technology
-
Teraoka Tohru
Tokyo Univ. Agriculture And Technol.(tuat) Tokyo Jpn
-
YAMAGUCHI Isamu
Microbial Toxicology Laboratory and Biodesign Research Group, RIKEN(The Institute of Physical and Chemical Research)
関連論文
- Antibiotic Susceptibility of Bifidobacterial Strains Distributed in the Japanese Market
- Suppressive Effects of Bifidobacterium breve Strain M-16V on T-Helper Type 2 Immune Responses in a Murine Model(Miscellaneous)
- cDNA Subtractive Cloning of Genes Expressed during Early Stage of Appressorium Formation by Magnaporthe grisea
- An Anti-hydrotactic Response and Solid Surface Recognition of Germ Tubes of the Rice Blast Fungus, Magnaporthe grisea
- Antiobesity Effects of Bifidobacterium breve Strain B-3 Supplementation in a Mouse Model with High-Fat Diet-Induced Obesity
- In Vitro Th1 Cytokine-Independent Th2 Suppressive Effects of Bifidobacteria
- Clinical Efficacy of Probiotic Bifidobacterium longum for the Treatment of Symptoms of Japanese Cedar Pollen Allergy in Subjects Evaluated in an Environmental Exposure Unit
- Oral Administration of an Immunostimulatory DNA Sequence from Bifidobacterium longum Improves Th1/Th2 Balance in a Murine Model
- Effect of Partially Hydrolyzed Guar Gum (PHGG) on the Bioaccessibility of Fat and Cholesterol
- Suppressive Effects of Dietary Fiber in Yogurt on the Postprandial Serum Lipid Levels in Healthy Adult Male Volunteers
- Identification of a Peptide Mimic of Bioactive Gibberellins with Affinity to GA 2-Oxidase
- EFFECT OF BRASSINOSTEROID ON PLANT DEFENSE RESPONSE AGAINST PATHOGENS
- EFFECTS OF SPECIFIC BRASSIONSTEROID (BR) BIOSYNTHESIS INHIBITOR (BRASSINAZOLE : Brz) ON PLANTS
- BRASSINOSTEROID BIOSYNTHESIS INHIBITOR : STRUCTURE, ACTIVITY AND TARGET SITES
- Effects of Mepanipyrim on Intracellular Trafficking: A Comparative Study on Its Effects on Exocytic and Endocytic Trafficking of Proteins, Sphingolipids, and Cholesterol
- Chlorophyll-deficient Mutants of Rice Demonstrated the Deletion of a DNA Fragment by Heavy-ion Irradiation
- Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea
- A catalytic subunit of cyclic AMP-dependent protein kinase, PKAC-1, regulates asexual differentiation in Neurospora crassa
- Expression in Cereal Plants of Genes That Inactivate Fusarium Mycotoxins(Biochemistry & Molecular Biology)
- Putative Homologs of SSK22 MAPKK Kinase and PBS2 MAPK Kinase of Saccharomyces cerevisiae Encoded by os-4 and os-5 Genes for Osmotic Sensitivity and Fungicide Resistance in Neurospora crassa(Biochemistry & Molecular Biology)
- Effects of Iprodione and Fludioxonil on Glycerol Synthesis and Hyphal Development in Candida albicans(Microbiology & Fermentation Technology)
- N-Cyanomethyl-2-chloroisonicotinamide Induces Systemic Acquired Resistance in Arabidopsis without Salicylic Acid Accumulation(Organic Chemistry)
- Chloroisonicotinamide Derivative Induces a Broad Range of Disease Resistance in Rice and Tobacco
- Structures of Oryzalides A and B, and Oryzalic Acid A, a Group of Novel Antimicrobial Diterpenes, Isolated from Healthy Leaves of a Bacterial Leaf Blight-resistant Cultivar of Rice Plant(Organic Chemistry)
- Pyrazolecarboxylic Acid Derivative Induces Systemic Acquired Resistance in Tobacco(Biochemistry & Molecular Biology)
- Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-Chain-Length Intermediates of β-Oxidation
- STUDIES ON SYSTEMIC ACQUIRED RESISTANCE INDUCED BY CHLOROISONICOTINATE
- Proteome Analysis of Wheat Lemma(Biochemistry & Molecular Biology)
- Inactivation of Blasticidin S by Bacillus cereus. VI. Structure and Comparison of the bsr Gene from a Blasticidin S-Resistant Bacillus cereus
- Nucleotide Sequence of the bsr Gene and N-Terminal Amino Acid Sequence of Blasticidin S Deaminase from Blasticidin S Resistant Eshcerichia coli TK121(Biological Chemistry)
- Cloning and Expression of a New Structural Gene for Blasticidin S Deaminase, a Nucleoside Aminohydrolase(Biological Chemistry)
- Production of Polyhydroxybutyrate by Polycistronic Expression of Bacterial Genes in Tobacco Plastid
- Enzyme Inhibitors to Increase Poly-3-hydroxybutyrate Production by Transgenic Tobacco(Biochemistry & Molecular Biology)
- Plastid Targeting of Polyhydroxybutyrate Biosynthetic Pathway in Tobacco
- Introduction of Bacterial Metabolism into Higher Plants by Polycistronic Transgene Expression(Biochemistry & Molecular Biology)
- Characterization of PBZ1,a Probenazole-inducible Gene, in Suspension-Cultured rice Cells.
- TRANSGENIC TOBACCO PRODUCING COPOLYMER IN PEROXISOMES
- Production of Biodegradable Polyester by a Transgenic Tobacco
- PROBENAZOLE, AN INDUCER OF BLAST FUNGUS RESISTANCE IN RICE PLANT, ACTIVATES SYSTEMIC ACQUIRED RESISTANCE IN TOBACCO AND ARABIDOPSIS
- A Wheat Xylanase Inhibitor Gene, Xip-I, but Not Taxi-I, Is Significantly Induced by Biotic and Abiotic Signals That Trigger Plant Defense
- New TAXI-type Xylanase Inhibitor Genes are Inducible by Pathogens and Wounding in Hexaploid Wheat
- Biotransformation of the Mycotoxin, Zearalenone, to a Non-estrogenic Compound by a Fungal Strain of Clonostachys sp.(Organic Chemistry)
- Effects of Partial Suppression of Ribosomal Protein S6 on Organ Formation in Arabidopsis thaliana(Biochemistry & Molecular Biology)
- EFFECTS OF TRICHOTHECENE MYCOTOXIN ON GROWTH AND MORPHOLOGY OF ARABIDOPSIS AND WHEAT
- Expression, Purification, and Characterization of Blasticidin S Deaminase (BSD) from Aspergillus terreus : The Role of Catalytic Zinc in Enzyme Structure
- A simple method for a mini-preparation of fungal DNA
- Induced Resistance to Rice Blast by Antagonistic Bacterium, Serratia marcescens Strain B2
- Effects of light conditions on prodigiosin stability in the biocontrol bacterium Serratia marcescens strain B2
- Identification of a Putative Vacuolar Serine Protease Gene in the Rice Blast Fungus, Magnaporthe grisea(Biochemistry & Molecular Biology)
- Studies on a Quantitative Analysis of Oryzalides and Oryzalic Acids in Rice Plants by GC-SIM
- Structures of Oryzalic Acid B and Three Related Compounds, a Group of Novel Antibacterial Diterpenes, Isolated from Leaves of a Bacterial Leaf Blight-Resistent Cultivar of Rice
- Novel C_-Kaurane Type of Diterpene (Oryzalide A), a New Antimicrobial Compound Isolated from Healthy Leaves of a Bacterial Leaf Blight-resistant Cultivar of Rice Plant(Organic Chemistry)
- イネ稲熱病に対する KC10017 の生物活性
- Targeted Gene Disruption of the Neuronal Calcium Sensor 1 Homologue in Rice Blast Fungus, Magnaporthe grisea(Biochemistry & Molecular Biology)
- A Novel Transformation System for Pyricularia oryzae : Adhesion of Regenerating Fungal Protoplasts to Collagen-coated Dishes
- Characterization of Endogenous Gibberellins in Dwarf Rice Mutants
- Comparison of cellobiose dehydrogenase and cellobiose : quinon oxydoreductase activities between Fusarium oxysporum f. sp. lycopersici, and its FCD1-disruptant
- Cloning of the pathogenicity-related gene FPD1 in Fusarium oxysporum f. sp. lycopersici
- Computer-Aided Modeling of Pentachlorophenol 4-Monooxygenase and Site-Directed Mutagenesis of Its Active Site
- AtFLA11, a Fasciclin-Like Arabinogalactan-Protein, Specifically Localized in Screlenchyma Cells
- Identification of Peptidyl Mimics of Bioactive Gibberellin Recognized by an Antibody
- Enzymatic Characterization of Scytalone Dehydratase Val75Met Variant Found in Melanin Biosynthesis Dehydratase Inhibitor (MBI-D) Resistant Strains of the Rice Blast Fungus
- Synthetic procedure for modifying the amine part of carpropamid : Changing 4-chlorophenethylamine to alkyl, alicyclic, and substituted phenylalkylamines
- Resistance buster compounds for MBI-D insensitive rice blast fungus : Inquiry on effective compounds among derivatives of MBI-D fungicides
- The Trichothecene Biosynthesis Regulatory Gene from the Type B Producer Fusarium Strains : Sequence of Tri6 and Its Expression in Escherichia coli
- Assessment of Gibberella fujikuroi mating type by PCR
- Features of Tri101,the Trichothecene 3-O-Acetyltransferase Gene, Related to the Self-defense Mechanism in Fusarium graminearum
- A Trichothecene Efflux Pump Encoded by TrilO2 in the Biosynthetic Gene Cluster of Fusarium graminearum
- Isolation of Gibberellin-binding Proteins by Aflinity Chromatography
- Control efficacy of validamycin A against Fusarium wilt correlated with the severity of phytotoxic necrosis formed on tomato tissues
- A104 Efficacy of Plant Activators for Banana Panama Disease caused by Fusarium oxysporum f.sp cubense
- Communication between rice plant and rice blast fungus at early infection stage
- Effect of Red Light Irradiance on Circumnutation of Arabidopsis thaliana Inflorescence Stems
- Antiobesity Effects of Bifidobacterium breve Strain B-3 Supplementation in a Mouse Model with High-Fat Diet-Induced Obesity
- Antibiotic Susceptibility of Bifidobacterial Strains Distributed in the Japanese Market
- Blasticidin S Deaminase Gene(BSD):a New Selection Marker Gene for Transformation of Arabidopsis thaliana and Nicotiana tabacum
- Population dynamics and pathogenic races of rice blast fungus, Magnaporthe oryzae in the Mekong Delta in Vietnam
- Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici, and its races
- Comparison of cellobiose dehydrogenase and cellobiose : quinon oxydoreductase activities between Fusarium oxysporum f. sp. lycopersici, and its FCD1-disruptant
- Induction of Resistance against Rice Bacterial Leaf Blight by 3-Chloro-1-methyl-1H-pyrazole-5-carboxylic Acid
- The Tomato Wilt Fungus Fusarium oxysporum f. sp. lycopersici shares Common Ancestors with Nonpathogenic F. oxysporum isolated from Wild Tomatoes in the Peruvian Andes