Comparison of cellobiose dehydrogenase and cellobiose : quinon oxydoreductase activities between Fusarium oxysporum f. sp. lycopersici, and its FCD1-disruptant

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概要

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• FCD1 in Fusarium oxypsorum f. sp. lycopersici (FOL) has been predicted to encode a cellobiose: quinone oxydoreductase (CBQ) (Kawabe et al. 2006). We compared the activity of cellobiose dehydrogenase (CDH) and CBQ in the cultural filtrate between a wild-type strain of FOL and an FCD1-disruptant by using 2, 6-dichloro-indophenol (DCIP)- and cytochrome c-assays. The results showed that DCIP-reducing activity (=CDH+CBQ activity) decreased in the FCD1-disruptant. On the other hand, cytochrome c-reducing activity (=CDH activity) was equivalent between the both strains. These confirmed that FCD1 encodes a CBQ. Moreover, FCD1 was found to distribute in various formae speciales of E oxysporum, suggesting that FCD1 is not responsible for determination of host-specificity in FOL.

• 日本土壌微生物学会の論文
• 2008-04-01

著者

• Teraoka T

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology (tuat)

• Teraoka T

  Tokyo Univ. Agriculture & Technol. Tokyo Jpn

• Teraoka Tohru

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Okabe Akiko

  Department Of Bioregulation And Biointeraction Graduate School Of Agriculture Tokyo University Of Ag

• Kimura M

  Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University

• Teraoka Tohru

  Graduate School Of Agriculture Tokyo University Of Agriculture And Technology

• Arie Tsutomu

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology (tuat)

• Arie Tsutomu

  Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology

• Kawabe Masato

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)

• Okabe Akiko

  Department Of Bioregulation And Biointeraction Graduate School Of Agriculture Tokyo University Of Agriculture And Technology (tuat)

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