Biocontrol activity in a nonpathogenic REMI mutant of Fusarium oxysporum f. sp. conglutinans and characterization of its disrupted gene
スポンサーリンク
概要
- 論文の詳細を見る
A nonpathogenic mutant (REMI10) of Fusarium oxysporum f. sp. conglutinans (FOC) was generated by restriction enzyme-mediated integration (REM1). REMI10 penetrated the cabbage root cortex much slower than FOC and never invaded the xylem through the endodermis. Previous treatment of cabbage seedlings with REMI10 reduced the disease incidence of yellows caused by FOC, suggesting that REMI10 has biocontrol activity. FOC invasion into root tissues was restricted when the seedling was treated with REMI10. The gene disrupted in REMI10 genome by plasmid insertion was identified, and designated sap1, which encodes a putative secreted aspartic proteinase. The sap1 disruptants showed no reduction in virulence toward cabbage, suggesting that SAP1 is not essential for pathogenicity in FOC.
- 日本農薬学会の論文
- 2010-04-21
著者
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Teraoka Tohru
Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology
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Arie Tsutomu
Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology
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Yoshida Takanobu
Environmental Biofunction Division National Institute For Agro-environmental Sciences
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YOSHIDA Takanobu
Environmental Biofunction Division, National Institute for Agro-Environmental Sciences (NIAES):United Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT)
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KAWABE Masato
Applied Biology for Plant Protection Research Unit, RIKEN
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MIYATA Yuichiro
Department of Bioregulatgion and Biointeraction, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT)
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TERAOKA Tohru
Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology (TUAT)
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