Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

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• N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signal molecules by many gram-negative bacteria. We have reported that Solibacillus silvestris, which was isolated from the potato leaf, has AHL-degrading activity. To identify the AHL-degrading gene, whole genome sequencing of S. silvestris StLB046 was performed by using pyrosequencing technology. As the result of the BLAST search, one predicted ORF (ahlS) showed slight similarity to AiiA-like AHL lactonase from Bacillus cereus group. Escherichia coil harboring the ahlS-expressing plasmid showed high AHL-degrading activity. The ahlS-cording region was also amplified by PCR from the other potato leaf-associated and AHL-degrading S. silvestris strains. Purified AhlS as a maltose binding fusion protein showed high AHL-degrading activity and catalyzes AHL ring opening by hydrolyzing lactones. In addition, expression of ahlS in plant pathogen Pectobacterium carotovorum subsp. carotovorum attenuated maceration of the potato slices. Our results suggest that AHL-degrading activity of ahlS might perform useful functions such as useful biocontrol agents.

著者

• Ikeda Tsukasa

  Department Of Applied Chemistry Utsunomiya University

• Morohoshi Tomohiro

  Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers

• Yoshida Takanobu

  Environmental Biofunction Division National Institute For Agro-environmental Sciences

• Someya Nobutaka

  National Agricultural Research Center For Hokkaido Region (narch) National Agricultural And Food Res

• Someya Nobutaka

  National Agricultural Research Center For Hokkaido Region (narch) National Agriculture And Food Rese

• Someya Nobutaka

  National Agricultural Research Center For Hokkaido Region (narch) National Agriculture And Food Rese

• Morohoshi Tomohiro

  Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers

• Tominaga Yoshiaki

  Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers

• Someya Nobutaka

  Hokkaido Agricultural Research Center (HARC), National Agriculture and Food Research Organization (N

• Someya Nobutaka

  Hokkaido Agricultural Research Center (harc) National Agriculture And Food Research Organization (na

• Someya Nobutaka

  Hokkaido Agricultural Research Center

• MOROHOSHI TOMOHIRO

  Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University

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