Identification and Characterization of N-Acylhomoserine Lactone-Acylase from the Fish Intestinal Shewanella sp. Strain MIB015

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概要

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• N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signal molecules by many gram-negative bacteria. We have reported that Shewanella sp. strain MIB015 degrades AHLs. In the present study, we cloned the aac gene from MIB015 by PCR with specific primers based on the aac gene in Shewanella oneidensis strain MR-1, which showed high homology with the known AHL-acylases. Escherichia coli expressing Aac showed high degrading activity of AHLs with long acyl chains. HPLC analysis revealed that Aac worked as AHL-acylase, which hydrolyzed the amide bond of AHL. In addition, expression of Aac in fish pathogen Vibrio anguillarum markedly reduced AHL production and biofilm formation. In conclusion, this study indicates that Aac might be effective in quenching quorum sensing of fish pathogens.

• 社団法人 日本農芸化学会の論文
• 2008-07-23

著者

• Kato Norihiro

  Department of Gene Diagnostic and Therapeutic, Research Institute, International Medical Center of J

• Kato Norihiro

  Dep. Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya Univ.

• IKEDA TSUKASA

  Department of Fermentation Technology, Hiroshima University

• Morohoshi Tomohiro

  Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya Unive

• EBATA ATSUSHI

  Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University

• NAKAZAWA SHIGEHISA

  Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University

• Ikeda Tsukasa

  Department Of Applied Chemistry Utsunomiya University

• Ikeda T

  Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.

• Ebata Atsushi

  Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University

• Kato Norihiro

  Department Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya Universi

• Morohoshi Tomohiro

  Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers

• Kato Norihiro

  Deparetment Of Gene Diagnositics And Theraputics Resarch Institute International Medical Center Of J

• Nakazawa Shigehisa

  Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University

• MOROHOSHI TOMOHIRO

  Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University

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