Diversity and Distribution of N-Acylhomoserine Lactone (AHL)-Degrading Activity and AHL-Lactonase (AiiM) in Genus Microbacterium
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概要
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N-Acylhomoserine lactone (AHL)-degrading enzyme, AiiM, was identified from the potato leaf-associated Microbacterium testaceum StLB037. In this study, we cloned eight aiiM gene homologues from other AHL-degrading Microbacterium strains. The similarity of the chromosomal locus of the aiiM gene is associated with the phylogenetic classification based on 16S rRNA. Degenerate PCR revealed that the aiiM gene was only conserved in AHL-degrading Microbacterium strains, but not in fifteen Microbacterium type strains or two Microbacterium isolates from other plants. These results suggested that the high level of AHL-degrading activity in Microbacterium strains was caused by the aiiM gene encoded on their chromosome.
- 2012-09-01
著者
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Ikeda Tsukasa
Department Of Applied Chemistry Utsunomiya University
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Morohoshi Tomohiro
Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers
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Someya Nobutaka
National Agricultural Research Center For Hokkaido Region (narch) National Agricultural And Food Res
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Someya Nobutaka
National Agricultural Research Center For Hokkaido Region (narch) National Agriculture And Food Rese
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Morohoshi Tomohiro
Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers
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Someya Nobutaka
Hokkaido Agricultural Research Center (harc) National Agriculture And Food Research Organization (na
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Someya Nobutaka
Hokkaido Agricultural Research Center
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Wang Wen-zhao
Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya University
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MOROHOSHI TOMOHIRO
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University
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