Identification of Quorum-Sensing Signal Molecules and the LuxRI Homologs in Fish Pathogen Edwardsiella tarda(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

スポンサーリンク

概要

• 論文の詳細を見る

• Edwardsiella tarda is a gram-negative bacterium that causes septicaemia in fish and serious damage to the aquaculture industry. The virulence factors of this pathogen and control mechanisms of the expression of virulence genes have not yet been clearly elucidated. A number of gram-negative pathogenic bacteria have a quorum-sensing system. These bacteria produce N-acyl-L-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. In this study, we found that E. tarda isolated from deceased flounder produces AHLs. Thin layer chromatography analysis indicated that the two kinds of AHL produced by E. tarda seemed to be N-hexanoyl-L-homoserine lactone (C_6-HSL) and N-heptanoyl-L-homoserine lactone (C_7-HSL). We have cloned and sequenced the quorum-sensing genes, luxl homolog (edwl) and luxR homolog (edwR). Edwl and EdwR showed high identity with CarI/CarR and ExpI/ExpR from Erwinia carotovora, respectively. SDS-PAGE analysis of extracellular proteins revealed that the expression of the 55-kDa protein, which was reported as a virulent-strain-specific protein, is controlled by AHLs. These results suggest that some virulence factors are regulated by the quorum-sensing system in E. tarda.

• 2004-10-25

著者

• Kato Norihiro

  Department of Gene Diagnostic and Therapeutic, Research Institute, International Medical Center of J

• Kanai Kinya

  Faculty of Fisheries, Nagasaki University

• Kato Norihiro

  Dep. Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya Univ.

• IKEDA TSUKASA

  Department of Fermentation Technology, Hiroshima University

• Morohoshi Tomohiro

  Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya Unive

• INABA TADANORI

  Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University

• Kanai Kinya

  Faculty Of Fisheries Nagasaki University

• Ikeda Tsukasa

  Department Of Applied Chemistry Utsunomiya University

• Ikeda T

  Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.

• Inaba Tadanori

  Department Of Applied Chemistry Faculty Of Engineering Utsunomiya University

• Kato Norihiro

  Department Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya Universi

• Morohoshi Tomohiro

  Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers

• Kato Norihiro

  Deparetment Of Gene Diagnositics And Theraputics Resarch Institute International Medical Center Of J

• IKEDA TSUKASA

  Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University

• MOROHOSHI TOMOHIRO

  Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University

関連論文

• 7 Anxiety and Poor Social Support are Independently Associated with Adverse Outcomes in Patients with Mild Heart Failure(Is Stress a Risk of Cardiovascular Disease?,Symposium 22 (SY-22) (I),The 73rd Annual Scientific Meeting of The Japanese Circulation So
• 7 Anxiety and Poor Social Support are Independently Associated with Adverse Outcomes in Patients with Mild Heart Failure(Is Stress a Risk of Cardiovascular Disease?,Symposium 22 (SY-22) (I),The 73rd Annual Scientific Meeting of The Japanese Circulation So
• Fas promoter region gene polymorphism is associated with an increased risk for myocardial infarction
• Characteristics and Outcomes of Hospitalized Patients With Heart Failure and Reduced vs Preserved Ejection Fraction : A Report From the Japanese Cardiac Registry of Heart Failure in Cardiology (JCARE-CARD)
• Gene-environmental interaction regarding alcohol-metabolizing enzymes in the Japanese general population
• Successful Isolation of a Chromosome 1 Region Affecting Blood Pressure, Stroke, and Glucose Tolerance In the Stroke-Prone Spontaneously Hypertensive Rat
• Detection of Type 1 Fimbrial Genes in Fish Pathogenic and Non-pathogenic Edwardsiella tarda Strains by PCR
• Proposition of a Feasible Protocol to Evaluate Salt Sensitivity in a Population-Based Setting
• Identification of Quantitative Trait Loci for Cardiac Hypertrophy in Two Different Strains of the Spontaneously Hypertensive Rat
• Evaluation of genetic susceptibility for cardiac hypertrophy with relation to hypertension, sexual dimorphism, and genetic make-up
• 8 Management of the "Real World" Patients with Heart Failure in Japan : JCARE and J-HOMECARE trial(Symposium 18 (SY-18) (H) How can we Make the Best Use of Japanese Evidences for Future Guidelines,Special Program,The 72nd Annual Scientific Meeting of the
• Cloning and Characterization of Pseudomonas putida Genes Encoding the Phosphate-Specific Transport System
• Anxiety and Poor Social Support are Independently Associated With Adverse Outcomes in Patients With Mild Heart Failure(Heart Failure)
• Evaluation of Pharmacogenetic Algorithm for Warfarin Dose Requirements in Japanese Patients
• Identification of Quorum-Sensing Signal Molecules and the LuxRI Homologs in Fish Pathogen Edwardsiella tarda(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• BM-P18 Inhibition of quorum sensing in gram-negative bacteria using AHL analogues and modified cyclodextrins(Section III Biomedical Engineering)
• BM-P17 Inhibitory control of quorum sensing using N-acylhomoserine lactone lactonase(Section III Biomedical Engineering)
• BM-P16 Phase-separated polymer networks that suit for inhibitory control of bacterial cell-to-cell communication(Section III Biomedical Engineering)
• Motility and the expression of the flagellin protein FliC are negatively regulated by quorum sensing in Edwardsiella tarda(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• N-acyl Homoserine Lactone-Producing or -Degrading Bacteria Isolated from the Intestinal Microbial Flora of Ayu Fish (Plecoglossus altivelis)
• Evaluation of Gene-Gene Interaction for Hypertension and Associated Metabolic Traits in Reciprocal Crossbreeds Derived from Chromosome-1 Congenic Rats
• Transcriptional Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11
• Development of a genetic transformation system for benzene-tolerant rhodococcus opacus strains
• Isolation and characterization of benzene-tolerant Rhodococcus opacus strains
• The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa
• Characterization of pEC01, a Novel Plasmid Required for Phosphate Taxis in Enterobacter cloacae IFO 3320
• Mutants of the Nematode Caenorhabditis elegans That Are Defective Specifically in Their Attraction to Cycloheximide(MISCELLANEOUS)
• A Method for Screening Polyphosphate-Accumulating Mutants Which Remove Phosphate Efficiently from Synthetic Wastewater(ENVIRONMENTAL BIOTECHNOLOGY)
• Cloning and Characterization of Extracellular Metal Protease Gene of the Algicidal Marine Bacterium Pseudoalteromonas sp. Strain A28(Microbiology & Fermentation Technology)
• Behavioral Responses of the Ciliated Protozoan Paramecium caudatum to 2,4-Dichlorophenoxyacetic Acid and Its Analogues
• Isolation and Characterization of cbbL and cbbS Genes Encoding Form I Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Large and Small Subunits in Nitrosomonas sp. Strain ENI-11(Microbiology & Fermentation Technology)
• The Synthesis of Optically Pure Enantiomers of N-Acyl-homoserine Lactone Autoinducers and Their Analogues
• Chemotaxis of the Nematode Caenorhabditis elegans toward Cycloheximide and Quinine Hydrochloride
• Magnetically Isotropic-Anisotropic Change of Thermally Responsive Polymer Gels
• Acceleration of Deswelling of Poly(N-isopropylacrylamide) Hydrogel by the Treatment of a Freeze-Dry and Hydration Process
• Diversity of Culturable Chitinolytic Bacteria from Rhizospheres of Agronomic Plants in Japan
• Isolation and Characterization of the Enterobacter cloacae cheR Mutant Defective in Phosphate Taxis
• Evaluation of Selected polymorphisms of the Mendelian Hypertensive Disease Genes in the Japanese Population
• Candesartan-Induced Gene Expression in Five Organs of Stroke-Prone Spontaneously Hypertensive Rats
• HapMap coverage for SNPs in the Japanese population
• Distribution of N-Acylhomoserine Lactone-Producing Fluorescent Pseudomonads in the Phyllosphere and Rhizosphere of Potato (Solanum tuberosum L.)
• Evaluation of Phosphate Removal from Water by Immobilized Phosphate-Binding Protein PstS
• Mutational Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11
• A Fluorescent Molecule-Recognition Sensor with a Protein as an Environmental Factor
• Remarkable Molecular Recognition of Dansyl-modified Cyclodextrin Dimer
• Syntheses and Molecular Recognition Abilities of 6-O-, 2-O-, and 3-O-Dansyl-γ-Cyclodextrins
• Formate Dehydrogenase Gene of Arabidopsis thaliana is Induced by Formaldehyde and Not by Formic Acid
• Intercalation of a Cationic Surfactant in the Interlayer Space of Smectites
• Saccharide Effect on the Deswelling Process of the Thermosensitive Poly(N-isopropylacrylamide) Ge
• OE-293 Genetic Analysis of Metabolic Syndrome in Rats(Genetics/Genetically Engineered Models 2 (H) : OE36)(Oral Presentation (English))
• Novel N-Acylhomoserine Lactone-Degrading Bacteria Isolated from the Leaf Surface of Solanum tuberosum and Their Quorum-Quenching Properties
• Deswelling Kinetics of a Porous Poly(N-isopropylacrylamide/methacrylic acid) Gel Prepared by Freeze-Drying
• Analysis of KRAP expression and localization, and genes regulated by KRAP in a human colon cancer cell line
• Identification and Characterization of N-Acylhomoserine Lactone-Acylase from the Fish Intestinal Shewanella sp. Strain MIB015
• Regulation of the Violacein Biosynthetic Gene Cluster by Acylhomoserine Lactone-Mediated Quorum Sensing in Chromobacterium violaceum ATCC 12472
• Diversity of Culturable Chitinolytic Bacteria from Rhizospheres of Agronomic Plants in Japan
• Confirmation of ALDH2 as a Major Locus of Drinking Behavior and of Its Variants Regulating Multiple Metabolic Phenotypes in a Japanese Population
• A Facile Transformation of Arabidopsis thaliana Using Ceramic Supported Propagation System
• Novel N-Acylhomoserine Lactone-Degrading Bacteria Isolated from the Leaf Surface of Solanum tuberosum and Their Quorum-Quenching Properties
• Streptococcus iniaeの免疫原性における莢膜の役割(英文)
• Exopolysaccharide Production Is Influenced by Sugars, N-Acylhomoserine Lactone, and Transcriptional Regulators RcsA and RcsB, but Does Not Affect Pathogenicity in the Plant Pathogen Pantoea ananatis
• Degradation of N-Acylhomoserine Lactone Quorum Sensing Signaling Molecules by Potato Root Surface-Associated Chryseobacterium Strains
• A Novel Oxidation Reaction and Its Application to Fmoc Solid Phase Synthesis of Therapeutic Photosensitizers
• Genetic Analysis in Human Hypertension
• Rate-Determining Step of Deswelling for Conventional and Macroporous Poly(N-isopropylacrylamide) Gels
• Studies of Gel Structure and Thermal Deswelling on Poly(N-isopropylacrylamide) Gel Treated by Freeze-Drying
• Degradation of N-Acylhomoserine Lactone Quorum Sensing Signaling Molecules by Potato Root Surface-Associated Chryseobacterium Strains
• Deswelling Kinetics oj Macroporous poly(N-isopropylacrylamide) Gel in Salt Solution
• Isolation and characterization of novel lipases from a metagenomic library of the microbial community in the pitcher fluid of the carnivorous plant Nepenthes hybrida(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Complete genome sequence and characterization of the N-acylhomoserine lactone-degrading gene of the potato leaf-associated Solibacillus silvestris(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Cell aggregation is negatively regulated by N-acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis SK-1(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Magnetically Isotropic–Anisotropic Change of Thermally Responsive Polymer Gels
• Genetic Diversity and Ecological Evaluation of Fluorescent Pseudomonads Isolated from the Leaves and Roots of Potato Plants
• The stroke-prone spontaneously hypertensive rat : still a useful model for post-GWAS genetic studies?
• Evaluation of genetic loci influencing adult height in the Japanese population
• Diversity and Distribution of N-Acylhomoserine Lactone (AHL)-Degrading Activity and AHL-Lactonase (AiiM) in Genus Microbacterium
• Inhibition of quorum sensing in gram-negative bacteria by alkylamine-modified cyclodextrins(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Production and Degradation of N-Acylhomoserine Lactone Quorum Sensing Signal Molecules in Bacteria Isolated from Activated Sludge
• Spacer-assisted catalytic action of imidazole-appended .GAMMA.-cyclodextrin.
• Phenazine antibiotic production and antifungal activity are regulated by multiple quorum-sensing systems in Pseudomonas chlororaphis subsp. aurantiaca StFRB508(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Detection of Pseudomonas aeruginosa quorum sensing signals in an infected ischemic wound : An experimental study in rats

もっと見る 閉じる

スポンサーリンク