Isolation and characterization of novel lipases from a metagenomic library of the microbial community in the pitcher fluid of the carnivorous plant Nepenthes hybrida(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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Members of the genus Nepenthes are carnivorous plants that use the pitfall method of insect capture as a supplementary nutritional source. We extracted metagenomic DNA from the microbial community found in the pitcher fluid of Nepenthes and constructed a plasmid-based metagenomic library. An activity-based screening method enabled the isolation of two lipase genes, lip1 and lip2. Both Lip1 and Lip2 belong to a novel family or subfamily of lipases and show lipase activities in acidic conditions, such as those found in pitcher fluid. This study was conducted under the assumption that the secreted Lip1 and Lip2 were capable of enzymatic activity in the acidic pitcher fluid.
著者
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Kato Norihiro
Dep. Of Advanced Interdisciplinary Sciences Graduate School Of Engineering Utsunomiya Univ.
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Kikuchi Noriko
Department Of Biochemistry Faculty Of Science Niigata University
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Ikeda Tsukasa
Department Of Applied Chemistry Utsunomiya University
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Ikeda T
Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.
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Morohoshi Tomohiro
Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers
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Kato Norihiro
Deparetment Of Gene Diagnositics And Theraputics Resarch Institute International Medical Center Of J
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Sato Shoko
Department Of General Internal Medicine The Jikei University School Of Medicine
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Kikuchi Noriko
Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers
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Oikawa Manabu
Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya Unive
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Oikawa Manabu
Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univers
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Oikawa Manabu
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Sato Shoko
Department Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya University
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MOROHOSHI TOMOHIRO
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University
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