Detection of Type 1 Fimbrial Genes in Fish Pathogenic and Non-pathogenic Edwardsiella tarda Strains by PCR
スポンサーリンク
概要
- 論文の詳細を見る
Presence of fimbrial genes among fish pathogenic and non-pathogenic strains of Edwardsiella tarda was investigated by polymerase chain reaction (PCR). Four primer sets for PCR were designed to detect the four genes (etfA, etfB, etfC, etfD) of the type 1 fimbrial gene cluster of E. tarda. All the four genes were successfully amplified with the four primer sets in all the pathogenic strains. On the other hand, any of etfA, etfB and etfC were not detected in 13 of 14 non-pathogenic strains. The results suggest that fimbriae play a role in the pathogenicity of E. tarda and that detection of etfA, etfB and etfC by PCR is useful to distinguish between pathogenic and non-pathogenic strains of E. tarda.
- 日本魚病学会,The Japanese Society of Fish Pathologyの論文
著者
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OSATOMI Kiyoshi
Faculty of Fisheries, Nagasaki University
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飯田 貴次
National Research Institute Of Aquaculture Fisheries Research Agency
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坂井 貴光
National Research Institute Of Aquaculture Fisheries Research Agency
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Sakai Takamitsu
National Research Institute of Aquaculture, Fisheries Research Agency
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Iida Takaji
National Research Institute of Aquaculture, Fisheries Research Agency
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Kanai Kinya
Faculty of Fisheries, Nagasaki University
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Iida Takaji
National Res. Inst. Of Aquaculture Fisheries Res. Agency
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長富 潔
Faculty Of Fisheries Nagasaki University
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Kanai Kinya
Faculty Of Fisheries Nagasaki University
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Osatomi Kiyoshi
Faculty Of Fisheries Nagasaki University
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Sakai Takamitsu
National Research Institute Of Aquaculture Fisheries Research Agency
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