A serine proteinase from the sarcoplasmic fraction of red sea bream Pagrus major is possibly derived from blood

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概要

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• Collagen degradation is known to be involved in the post mortem tenderization of fish muscle. A serine proteinase that is assumed to be related to collagen degradation after fish death was purified from the sarcoplasmic fraction of red sea bream Pagrus major by ammonium sulfate fractionation and column chromatography on Sephacryl S-300, Q Sepharose and Phenyl Sepharose CL-4B. The enzyme hydrolyzed gelatin and was obtained as a protein band of approximately 38 kDa upon sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions. The N-terminal amino acid sequence of the enzyme was determined for 32 residues. A protein that had the same N-terminal amino acid sequence as the enzyme for ten residues was purified from the serum of red sea bream and showed the same characteristics as the enzyme. Therefore, it is suggested that the serine proteinase migrates from the blood to muscle and degrades muscle proteins after the death of the fish.

• Springer Japanの論文
• 2009-11-01

著者

• BAE Inwoo

  Graduate School of Science and Technology, Nagasaki University

• OSATOMI Kiyoshi

  Faculty of Fisheries, Nagasaki University

• YOSHIDA Asami

  Graduate School of Science and Technology, Nagasaki University

• TACHIBANA Katsuyasu

  Faculty of Fisheries, Nagasaki University

• HARA Kenji

  Faculty of Fisheries, Nagasaki University

• SONODA Hiroko

  Faculty of Fisheries, Nagasaki University

• CAO Min-Jie

  College of Biological Engineering, The Key Laboratory of Science and Technology for Aquaculture and

• Osako K

  Department Of Food Science And Technology Tokyo University Of Marine Science And Technology

• Osatomi K

  Faculty Of Fisheries Nagasaki University

• Osatomi Kiyoshi

  Graduate School Of Marine Sciences And Engineering Nagasaki University

• Cao Minjie

  College Of Biological Engineering The Key Laboratory Of Science And Technology For Aquaculture And F

• Bae Inwoo

  Graduate School Of Science And Technology Nagasaki University

• Hara Kenji

  Faculty Of Fisheries Nagasaki University

• Hara Keniji

  Faculty Of Fisheries Nagasaki University

• Cao Min-jie

  College Of Biological Engineering The Key Laboratory Of Science And Technology For Aquaculture And F

• Sonoda Hiroko

  Faculty Of Fisheries Nagasaki University

• Yoshida Asami

  Graduate School Of Science And Technology Nagasaki University

• Osatomi Kiyoshi

  Faculty Of Fisheries Nagasaki University

• Osatomi Kiyoshi

  Graduate School Of Marine Science And Engineering Nagasaki University

• Cao Min-jie

  Graduate School Of Marine Science And Engineering Nagasaki University

• Tachibana Katsuyasu

  Laboratory Of Fishery Nutritional Sciences Graduate School Of Marine Science And Engineering Nagasak

• Tachibana Katsuyasu

  Faculty Of Fisheries Nagasaki University

• Hara Kenji

  Faculty Of Agriculture Kyushu University

• Cao Min-jie

  College Of Biological Engineering The Key Laboratory Of Science And Technology For Aquaculture And F

• Yoshida Asami

  Graduate School Of Fisheries Science And Environmental Studies Nagasaki University

• Hara Kenji

  Graduate School Of Fisheries Science And Environmental Studies Nagasaki University

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