The Trichothecene Biosynthesis Regulatory Gene from the Type B Producer Fusarium Strains : Sequence of Tri6 and Its Expression in Escherichia coli
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概要
- 論文の詳細を見る
A genomic DNA fragment containing Tri6,a transcription activator gene of trichothecene biosynthesis, was cloned by vectorette PCR from Fusarium graminearum F15,which produces type B trichothecene, deoxynivalenol. The nucleotide sequence of the gene showed 84% of identity to that of the type A trichothecene producer Fusarium sporotrichioides NRRL 3299,but the sequence around the initiation codon was not highly conserved between these producers. Based on the upstream and downstream sequences of the coding region of F. graminearum, Tri6 could be amplified by PCR from other type B trichothecene producers. Tri6 appeared to be expressed for only a limited period prior to the toxin production.
- 社団法人日本農芸化学会の論文
- 1999-11-23
著者
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YAMAGUCHI Isamu
Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN)
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Kimura M
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamaguchi I
Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie
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Yamaguchi Isamu
Laboratory For Remediation Research Plant Science Center Riken
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Yamaguchi I
Riken The Institute Of Physical And Chemical Research
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KIMURA Makoto
Microbial Toxicology Laboratory and Biodesign Research Group, RIKEN(The Institute of Physical and Ch
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Yamaguchi Isamu
Microbial Toxicology Laboratory Riken:(present Address)environmental Plant Research Group Plant Scie
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Kawabe Masato
United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology (tuat)
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Yamaguchi I
Riken (the Institute Of Physical And Chemical Research) Plant Science Center
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MATSUMOTO Gentaro
Microbial Toxicology Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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WUCHIYAMA Junko
Microbial Toxicology Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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SHINGU Yoshinori
Microbial Toxicology Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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YONEYAMA Katsuyoshi
Plant Pathology Laboratory, Faculty of Agriculture, Meiji University
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Wuchiyama Junko
Microbial Toxicology Laboratory Riken
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Matsumoto Gentaro
Microbial Toxicology Laboratory Riken (the Institute Of Physical And Chemical Research):plant Pathol
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Yoneyama K
Shinshu Univ. Nagano Jpn
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Shingu Y
Microbial Toxicology Laboratory Riken (the Institute Of Physical And Chemical Research):plant Pathol
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Kimura Makoto
Microbial Toxicology Laboratory And Biodesign Research Group Riken(the Institute Of Physical And Che
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YAMAGUCHI Isamu
Microbial Toxicology Laboratory and Biodesign Research Group, RIKEN(The Institute of Physical and Chemical Research)
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