A Method for the Simultaneous Determination of 3T3-L1 Adipocyte Metabolites by Liquid Chromatography/Mass Spectrometry Using [13C]-stable Isotopes
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概要
- 論文の詳細を見る
A useful method employing liquid chromatography mass spectrometry (LC/MS) and a stable isotope was developed for simultaneous examination of major metabolism in adipocytes, de novo fatty acid synthesis, glycerol output, and glucose uptake with high sensitivity. The addition of thiazolidinediones, potent agonists of peroxisome proliferator-activated receptors-γ, for 10 d increased glucose uptake in a dose-dependent manner. Fatty acid (FA) synthesis increased at low concentrations of thiazolidinediones (TZDs) and decreased at high concentrations. It is important to assess adipocytes from various examples of metabolism, because each example of adipocyte metabolism is directly related to obesity or metabolic syndrome in various ways. The technique makes metabolic examination easier than conventional methods by means of radioisotopes and makes it possible to identify metabolites and to apply them in biomarker screening.
著者
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NAGAI Hiroyuki
Laboratory for Molecular Function of Food, Division of Food Science and Biotechnology, Graduate Scho
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GOTO Tsuyoshi
Laboratory for Molecular Function of Food, Division of Food Science and Biotechnology, Graduate Scho
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TAKAHASHI Nobuyuki
Laboratory for Molecular Function of Food, Division of Food Science and Biotechnology, Graduate Scho
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KAWADA Teruo
Laboratory for Molecular Function of Food, Division of Food Science and Biotechnology, Graduate Scho
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Takahashi Nobtaka
Department Of Agricultural Chemistry The University Of Tokyo:(presnet Addresses) The Institute Of Ph
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Takahashi N
Graduate School Of Agriculture Kyoto Univ.
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KUSUDOH Tatsuya
Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate Schoo
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DEYASHIKI Yoshihiro
Department of Drug Metabolism, Faculty of Pharmaceutical Sciences, Suzuka University of Medical Scie
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ESAKA Yukihiro
Laboratory of Pharmaceutical Analytical Chemistry, Department of Bioactive Molecules, Gifu Pharmaceu
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Kawada Teruo
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
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Nagai Hiroyuki
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
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Goto Tsuyoshi
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
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