A comparison of osteogenesis-related gene expression of mesenchymal stem cells during the osteoblastic differentiation induced by Type-I collagen and/or fibronectin
スポンサーリンク
概要
- 論文の詳細を見る
Mesenchymal stem cells (MSCs) contain multipotent cells and differentiate into osteoblasts, chondrocytes, and adiphocytes. Recently, MSCs have been used clinically in a tissue-engineering concept, but cell activity varies. It was found that the characteristics of extracellular matrices, type Tcollagen and/or fibronectin matrices, induced the osteoblastic differentiation of MSCs. Six weeks after cells were cultured with type I collagen and fibronectin, they formed mineralized tissue six times higher than other matrices. In this study, using "real time RT-PCR," we assessed the expression of osteogenesis-related genes (alkaline phosphatase, BMP-2, osteocalcin, and cbfa-1) during osteoblastic differentiation by measuring their mRNA, quantitatively. The expression of the alkaline phosphatase gene increased time-dependently during osteoblastic differentiation until day 12, but expression induced by type I collagen and type I collagen and fibronectin decreased gradually. The expression of osteocalcin and the BMP-2 gene increased time-dependently; osteocalcin gene expression induced by type I collagen and fibronectin was about 6.2 times higher than non-induced cells. Expression of the cbfa-1 gene was different from that of type I collagen, fibronectin, and non-induced cells from day 12 and increased time-dependently. These findings were supported by alkaline phosphatase staining and immunofluoresence with an osteopontin antibody.
- 日本大学の論文
著者
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Ueda Minoru
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
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NAGASAKA TETSURO
Department of Pathology, Nagoya University School of Medicine
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Nagasaka Tetsuro
Department Of Laboratory Medicine Nagoya University Graduate School Of Medicine Nagoya University Sc
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YAMADA Yoichi
Department of Electrical and Electronic Engineering, Yamaguchi University
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Ueda Minoru
Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine
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Nagasaka Tetsuro
Department Of Clinical Laboratory Of Nagoya University Hospital
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Yamada Yoichi
Center For Genetic And Regenerative Medicine Nagoya University School Of Medicine
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OZAWA Ryotaro
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
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Ozawa Ryotaro
Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine
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Ueda Minoru
Department Of Chemistry Faculty Of Science Tohoku University
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Ueda Minoru
Department Of Applied Chemistry Aichi Institute Of Technology
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Yamada Yoichi
Department Of Cellular Physiology And Signal Transduction Sapporo Medical University School Of Medic
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Ueda Minoru
Department 1 Product Development Division 1 Motorcycle R&d Center Honda R&d Co. Ltd.
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