Expression of Odontoblastic-Related Genes in Human Dental Follicle Cells, Dental Pulp Stem Cells, and Oral Mucosal Cells
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概要
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In the current studies, we examined the ability of human dental follicle cells (DFCs), dental pulp stem cells (DPSCs), and oral mucosal cells (OMCs) to form dentin by analyzing their expression of the odontoblast-specific genes, dentin sialophosphoprotein (DSPP) and dentin sialoprotein (DSP), and the mineralization-associated genes, alkaline phosphatase (ALP) and dentin matrix protein-1 (DMP-1). Phase contrast microscopy showed that DFCs, DPSCs, and OMCs exhibited spindle-shaped and fibroblastic morphologies. Also, more bone nodules were formed in DFCs than in DPSCs, whereas bone nodules were not found in OMCs. Analysis of gene expression by real-time reverse transcriptase-polymerase chain reaction showed a time-dependent increase in the expression of DSPP in differentiating DFCs and DPSCs, but almost no expression in OMCs. The expression of DSPP in DFCs was approximately 4-fold higher than in DPSCs after 15 days in culture, and, almost 6-fold higher after 27 days. In addition, the expression of DSP, DMP-1 and ALP was observed in both DFCs and DPSCs, with a slow increase until 15 days of differentiation, and, after 21 days, there was a rapid increase in the expression of these genes in DFCs. In contrast, expression of these genes was almost undetectable in OMCs and in undifferentiated DFCs, DPSCs and OMCs. These results suggest that, compared to DPSCs, DFCs have a superior capacity for dentin formation and mineralization and that they may be useful for the regeneration of dentin or tooth tissues using tissue engineering technology.
- 日本大学の論文
著者
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Ueda Minoru
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
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TAKAHASHI MAKOTO
Department of Food Science and Technology, Kyoto University
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Ueda Minoru
Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine
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Yamada Yoichi
Center For Genetic And Regenerative Medicine Nagoya University School Of Medicine
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Ito Kenji
Department of Research and Development, Yoshida Pharmaceutical Co., Ltd.
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OHYA Morimichi
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
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OZAWA Ryotaro
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
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Ozawa Ryotaro
Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine
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Ohya Morimichi
Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine
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Ueda Minoru
Department Of Chemistry Faculty Of Science Tohoku University
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Ueda Minoru
Department Of Applied Chemistry Aichi Institute Of Technology
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Takahashi Makoto
Department Of Coloproctological Surgery Juntendo University Faculty Of Medicine
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Ito Kenji
Department Of Oral And Maxillofacial Surgery Nagoya University Graduate School Of Medicine
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Ito Kenji
Department Of Anesthesiology Tokai University School Of Medicine
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Ueda Minoru
Department 1 Product Development Division 1 Motorcycle R&d Center Honda R&d Co. Ltd.
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Takahashi Makoto
Department Of Applied Chemistry College Of Engineering Chubu University
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