Continuous Chitosan Hydrolyzate Production by Immobilized Chitosanolytic Enzyme from Enterobacter sp.G-1
スポンサーリンク
概要
- 論文の詳細を見る
Chitosanolytic enzymes from Enterobacter sp.G-1 were immobilized on various carriers to continuously hydrolyze chitosan. Four different carriers were tested : FE-3901 (strong basic anion exchange resin, ionic binding), glutaraldehyde-treated FE-4612 (weak basic anion exchange resin, cross-linking), Chitopearl (chitosan beads), and alginate calcium. Glutaraldehyde-treated FE-4612 and Chitopearl immobilized more protein than the others. The enzyme immobilized on FE-3901 had the greatest activity. The activity of enzyme immobilized on FE-3901 decreased rapidly when exposed to a continuous flow of 1% chitosan. The enzyme immobilized with Chitopearl retained more than 50% of its original activity after 17 days, and the activity was fully restored by re-immobilization.
- 社団法人日本農芸化学会の論文
- 1992-10-23
著者
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Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
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KAWAMUKAI Makoto
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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MATSUDA Hideyuki
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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FUKUMOTO Ikuo
Department of Biochemistry and Biotechnology, Faculty of Life and Environmental Science
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YAMASAKI Yukikazu
Institute of Industrial Science and Technology in Shimane Prefecture
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Fukumoto Ikuo
Department Of Biochemistry And Biotechnology Faculty Of Life And Environmental Science
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Kumagai Naoki
Applied Biological Sciences Faculty Of Agriculture Shimane University
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Oowatari Y
Shimane Univ. Matsue Jpn
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NAKAGAWA Tsuyoshi
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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Morita K
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Kawamukai M
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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Matsuda H
Department Of Applied Bioscience And Biotechnology Faculty Of Life And Environmental Science Shimane
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YAMASAKI Yukikazu
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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FUKUMOTO Ikuo
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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OHTA Yukari
Applied Biological Sciences, Faculty of Agriculture, Shimane University
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Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
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