The Mechanism of Inactivation of Bacteriophage ØX174 by Anthracycline Antibiotics
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概要
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The interaction of some anthracycline antibiotics (adriamycin, daunomycin, aclacinomycin-A) with bacteriophage øX174 was investigated. Adriamycin and daunomycin inactivated the infectivity of both free øX174 phage and naked single-stranded øX174 DNA without DNA strand scission, but aclacinomycin-A did not show this action. The phage inactivation reaction was reversibly inhibited by superoxide dismutase, catalase or other oxygen radical scavengers. The inactivation of øX174 by adriamycin and aclacinomycin-A was stimulated by the addition of Cu2+, while the øX174 inactivation by daunomycin was inhibited by the addition of Cu2+. The øX174 inactivation by adriamycin and aclacinomycin-A in the presence of Cu2+ was caused by degradation of DNA, and this inactivation reaction was inhibited irreversibly by oxygen radical scavengers. These results indicate that anthracycline antibiotics bind to ΦX174 DNA in the form of free radicals and that during the auto-oxidation of these antibiotics in the presence of Cu2+, oxygen radicals were generated to cause the degradation of øX174 DNA.
- 社団法人 日本農芸化学会の論文
著者
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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TANAKA Akira
Laboratry of Drug Analysis, Showa College of Pharmaceutical Sciences
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MORITA Junji
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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