Involvement of a Cell Envelope Component of Escherichia coli in the Early Stages of Infection with Bacteriophage φX174
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概要
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Envelope fraction I prepared from a φX174 sensitive host, KD4301, showed a strong eclipsing activity, while the lipopolysaccharide (LPS) fraction showed a weak activity. The eclipsing activity in envelope fraction I was sensitive to heat treatment, while that in the LPS fraction was insensitive. When the complete phage particles (114S) were treated with envelope fraction I, the eclipsed particles (70S) and a rapidly sedimenting component were obtained, but when they were treated with LPS, only 70S eclipsed particles were obtained. Electron microscopic observation showed that there were two types of eclipsed particles formed on treatment with fraction I; in one of them phage DNA was extruded from the phage particles as a thick bundle, and in the other more than 95% of the phage DNA was extruded from the phage particles. The rapidly sedimenting component was the membrane-eclipsed particle complex. LPS gave only one type of eclipsed particles in which DNA was extruded as a thick bundle. These results indicate that a heat labile component in the cell envelopes other than LPS is involved in the extrusion of φX174 DNA.
- 社団法人 日本農芸化学会の論文
著者
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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MANO Yoshihiro
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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KAWABE Tatsuya
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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YAZAKI Kazumori
Hepatitis Division, Tokyo Metropolitan Institute of Medical Science
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