Effect of Polyethylene Glycol in Plasmid DNA Solution on Transformation of CaCl2-Treated Escherichia coli Cells
スポンサーリンク
概要
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Transformation of pBR322 DNA to Escherichia coli cells treated with CaCl2 was stimulated by addition of 8% polyethylene glycol (PEG) 600 to the DNA solution. Prolonged incubation of the competent cells in chilled CaCl2 solution (Dargert and Ehrlich, 1979) raised the transformation efficiency by a factor of 5. This prolonged incubation did not inhibit the stimulus effect of PEG. When PEG 600 or PEG 1, 000 was added to the mixture of plasmid pBR322 DNA to a final concentration of 2% and the competentcells were incubated at 0°C for 24 hr, weroutinely obtained over 107 transformants per μg of pBR322 DNA, and the competent cells transformed comprised 24% of the viable cells.
著者
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Himeno Michio
Laboratory Of Applied Molecular Biology Department Of Applied Biochemistry Osaka Prefecture Universi
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HIMENO Michio
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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HANAOKA Yuhji
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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SHIBATA Toshihiro
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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KAWAHARA Yoshio
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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