Studies on the Functional Relationship between φX174 and SI3 Coded Proteins Using an In Vitro DNA Synthesis System
スポンサーリンク
概要
- 論文の詳細を見る
The functional relationship between bacteriophage φX174 and SI3 gene products was investigated using an in vitro DNA synthesis system in two stages: (a), replication of the closed circular duplex form DNA (RF to RF) and (b) synthesis of single-stranded DNA from RF and formation of the intermediate 50S complex in phage morphogenesis (RF to 50S complex). Partially purified gene A products of φX174 and S13 were able to function reciprocally in the stage from RF to RF replication. In the stage of RF to 50S complex, extracts from Escherichia coli infected with φX174 catalyzed the formation of 50S complex from S13 RF DNA. Extracts from E. coli infected with SI3 also had the ability to catalyze the formation of 50S complex from φX174 RF DNA. Thus, all gene products required for DNA replication and phage morphogenesis were found to function reciprocally between φX174 and S13.
- 社団法人 日本農芸化学会の論文
著者
-
Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
-
Sakai Hiroshi
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
-
WATABE Kounosuke
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
-
KUGA Tetsuro
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
関連論文
- Structural Analysis of Repetitive DNA Sequences in the Goat Growth Hormone Gene Region(Biological Chemistry)
- Cloning and Sequencing of Goat Growth Hormone Gene(Biological Chemistry)
- Tissue- and Development-specific Expression of Goat Insulin-like Growth Factor-I (IGF-I) mRNAs
- Dynamic Aspects in the Expression of the Goat Insulin-like Growth Factor-I (IGF-I)Gene : Diversity in Transcription and Post-transcription
- Binding of an Engineered 130-kDa Insecticidal Protein of Bacillus thuringiensis var.israelensis to Insect Cell Lines
- Importance of the Central Region of 130-kDa Insecticidal Proteins of Bacillus thuringiensis var. israelensis for Their Activity in Vivo and in Vitro
- Effects of Bacillus thuringiensis var.israelensis 20-kDa Protein on Production of the Bti 130-kDa Crystal Protein in Escherichia coli
- Expression of the Two 130-kDa Protein Genes of Bacillus thuringiensis var. israelensis in Bacillus subtilis(Biological Chemistry)
- Insecticidal Activity of a Peptide Containing the 30th to 695th Amino Acid Residues of the 130-kDa Protein of Bacillus thuringiensis var. israelensis(Biological Chemistry)
- Insecticidal Activity of the Products of Two 130-kDa Protein Genes(Biological Chemistry)
- Cloning and Nucleotide Sequences of the Two 130 kDa Insecticidal Protein Genes of Bacillus thuringiensis var. israelensis(Biological Chemistry)
- Genomic Structure and 5'-Flanking Sequences of Rat N-Acetylglucosaminyltransferase I Gene and Regulatory Role of Its Transcriptional Diversity(Biochemistry & Molecular Biology)
- Characterization of Rat N-Acetylglucosaminyltransferase I Expressed in Esherichia coli
- Cloning of a cDNA Encoding N-Acetylglucosaminyltransferase I from Rat Liver and Analysis of Its Expression in Rat Tissues
- Expression of Human P-Glycoprotein in Yeast Cells : Effects of Membrane Component Sterols on the Activity of P-Glycoprotein(Biological Chemistry)
- Production of a Site Specifically Cleavable P-Glycoprotein-β-galactosidase Fusion Protein(Biological Chemistry)
- The Effects of Metal Ions on the DNA Damage Induced by Hydrogen Peroxide(Biological Chemistry)
- Alternative Secondary Structures in the Phage G4 Origin of the Complementary DNA Strand Synthesis : Effects of NaCl Concentration on the Bleomycin-DNA Interaction
- Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate (2):Importance of the P2 and P4 Residues
- Transient Expression of Goat Growth Hormone Gene in Poplar (Populus alba L.) Protoplasts : A Quick Method for Detection of Foreign Gene Expression in mRNA Level
- Cleavage Specificity of Coxsackievirus 3C Proteinase for Peptide Substrate
- Mutational Analysis of the Putative Substrate-binding Site of 3C Proteinase of Coxsackievirus B3
- Expression and Purification of Recombinant 3C Proteinase of Coxsackievirus B3
- Nucleotide Sequence of the Glycerol-3-phosphate Dehydrogenase Gene of Escherichia coli and Regulation by the cAMP-CRP Complex(Biological Chemistry)
- Plasmids and Insecticidal Activity of Delta-endotoxin Crystals from Bacillus thuringiensis var. israelensis
- Mechanism of Action of Bacillus thuringiensis Insecticidal Delta-endotoxin on Insect Cells in Vitro
- Functional Distinction among Structural Subsections in the Specific Priming Signal for DNA Replication of the Broad Host-Range Plasmid RSF1010
- GROWTH REGULATION BY cAMP IN ESCHERICHIA COLI
- Effects of cya and cid Mutations on Cell Growth in Escherichia coli(Biological Chemistry)
- Effect of Polyethylene Glycol in Plasmid DNA Solution on Transformation of CaCl2-Treated Escherichia coli Cells
- Effects of Detergents and Divalent Cations on the Functioning of Cell Envelopes of Escherichia coli in the Early Stages of Infection with Bacteriophage øX174
- DNA Replication of IncQ Broad-host-range Plasmids in Gram-negative Bacteria
- A Simple Method for Isolation of Chloroplast DNA from Marchantia polymorpha L. Cell Suspension Cultures
- In Vitro DNA Synthesis by Chloroplasts Isolated from Marchantia polymorpha L. Cell Suspension Cultures
- DNA Strand Breakage In Vitro by Autoxidized Unsaturated Fatty Acids
- The Mechanism of Inactivation of Bacteriophage ØX174 by Anthracycline Antibiotics
- Induction of Strand Breakage in φX174 RFI DNA by Aminosugar Derivatives
- Molecular Cloning of Promoters Functional in Escherichia coli from Chloroplast DNA of a Liverwort, Marchantia polymorpha
- Induction of Single Strand Scission in φX174 RFI DNA by D-Isoglucosamine
- Inactivation of Escherichia coli Phages by Sugar Phosphates
- Actions of Mitomycin C Reduced with Sodium Borohydride on Bacteriophage ΦX174 and Its Single- and Double-stranded DNAs
- Involvement of a Cell Envelope Component of Escherichia coli in the Early Stages of Infection with Bacteriophage φX174
- DNA Products in In Vitro DNA Synthesis Using Bacteriophage ΦX174 Single-stranded DNA
- Studies on the Functional Relationship between φX174 and SI3 Coded Proteins Using an In Vitro DNA Synthesis System