Dynamic Aspects in the Expression of the Goat Insulin-like Growth Factor-I (IGF-I)Gene : Diversity in Transcription and Post-transcription
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概要
- 論文の詳細を見る
Goat IGF-I (gIGF-I) cDNA was cloned using the reverse transcriptase-polymerase chain reaction (RT-PCR). The cDNA, which was homologous to rat class 1 IGF-I cDNA, was 969bp long. From the open reading frame found in it, we predicted a 154 amino acid protein consisting of a 49 amino acid signal peptide, a 70 amino acid mature IGF-I peptide, and a 35 amino acid E domain (the COOH-terminal peptide). From the gIGF-I gene, we isolated and sequenced some segments containing four exons that encompassed the entire gIGF-I cDNA sequence. Goat liver RNA was analyzed by RT-PCR, and the nucleotides of the RT-PCR products were sequenced and checked with the nucleotide sequences of the segments from the gIGF-I gene. The gene had three leader exons (1W, 1, and 2, from upstream to downstream) and was transcribed into three kinds of mRNAs (classes 1W, 1, and 2). Another RNA species was detected by RT-PCR analysis of exon 1W. We sequenced it and found that in this transcript, the 3'-Portion of exon 1 was inserted between exons 1W and 3, resulting in class 1W-1del. mRNA. That is to say, the gIGF-I gene had three leader exons and four kinds of mature mRNA.
- 社団法人日本農芸化学会の論文
- 1995-01-23
著者
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Komano T
Kyoto Univ. Kyoto Jpn
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Komano Tohru
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Komano Tohru
京都大学
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Yamano Yoshiaki
Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University
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Aoki H
Iwate Univ. Iwate Jpn
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Aoki Hideyuki
Research Laboratory Ikeda Food Research Co. Ltd.
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Aoki H
Ikeda Food Res. Co. Ltd. Hiroshima Jpn
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MIKAWA Satoshi
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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SAKAI Hiroshi
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Mikawa S
National Inst. Agricultural Sci. Tsukuba
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Mikawa Satoshi
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Yoshikawa Gen-Ichi
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Aoki Hideyuki
Laboratory of Biochemistry, Department of Agricultural Chemistry, Kyoto University
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Sakai H
Okayama Univ. Okayama Jpn
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Mikawa Satoshi
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Yamano Yoshiaki
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Yamano Yoshiaki
Laboratory Of Metabolic Biochemistry Department Of Biochemistry And Biotechnology Faculty Of Agricul
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Sakai H
Department Of Bioscience And Biotechnology Okayama University
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Sakai H
Department Of Pharmacology Nagasaki University Shool Of Dentistry
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Komano T
Laboratory Of Cellular Biochemistry Division Of Applied Life Sciences Graduate School Of Agriculture
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Sakai Hiroshi
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Yoshikawa Gen-ichi
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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