Isolation of 2-Methylisocitrate Dehydratase, a New Enzyme Serving in the Methylcitric Acid Cycle for Propionate Metabolism, from Yallowia lipolytica
スポンサーリンク
概要
- 論文の詳細を見る
2-Methylisocitrate dehydratase (EC 4. 2. 1. - ; (2S, 3R)-3-hydroxybutane-1, 2, 3-tricarboxylate hydrolyase), a new enzyme functioning in the methylcitric acid cycle of propionate metabolism, was found in cells of Yallowia lipolytica, separated from the usual aconitase (EC 4. 2. 1. 3), and purified about 120-fold to homogeneity as judged electrophoretically. The new enzyme catalyzed a reversible reaction between 2-methylisocitrate and 2-methyl-cis-aconitate, but was inactive toward 2-methylcitrate, citrate, cis- or trans-aconitate, isocitrate, or the other hydroxy-acids tested. Equilibrium was reached at about 92% of 2-methylisocitrate, with the rest as 2-methyl-cis-aconitate. The isolated aconitase also catalyzed the reversible reaction and its K_m for 2-methylisocitrate was 120μM, but that of the new enzyme was 18μM.
- 社団法人日本農芸化学会の論文
- 1995-10-23
著者
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Aoki H
Iwate Univ. Iwate Jpn
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Aoki Hideyuki
Research Laboratory Ikeda Food Research Co. Ltd.
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Aoki H
Ikeda Food Res. Co. Ltd. Hiroshima Jpn
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Uchiyama Hiroo
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Uchiyama H
National Inst. Environmental Studies Ibaraki Jpn
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Uchiyama H
National Institute For Environmental Studies
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Uchiyama Hiroo
National Institute Of Environmental Studies
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Uchiyama Hiroo
National Institute For Environmental Studies
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Uchiyama Hiroo
Water And Soil Environment Division National Institute For Enviromental Studies
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TABUCHI Takeshi
Institute of Applied Biochemistry, University of Tsukuba
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Tabuchi T
Institute Of Bioscience And Biotechnology Aomori University
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Tabuchi Takeshi
Institute Of Applied Biochemistry The University Of Tsukuba
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Umetsu H
Japan Women's Univ. Tokyo Jpn
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Umetsu Hironori
Institute Of Bioscience And Biotechnology Aomori University
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Aoki Hiroyuki
Institute of Applied Biochemistry, University of Tsukuba
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Aoki Hiroyuki
Institute of Applied Biochemistry, The University of Tsukuba
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