Survival and Impact of Genetically Engineered Pseudomonas putida Harboring Mercury Resistance Gene in Aquatic Microcosms
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概要
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The survival of wild-type and genetically engineered Pseudomonas putida PpY101 that contained a recombinant plasmid pSR134 conferring mercury resistance were monitored in aquatic microcosms. We used lake, river, and spring water samples. The density of genetically engineered and wild-type P. putida decreased rapidly within 5 days (population change rate k -0.87〜-1.00 day^<-1>), then moderately after 5 to 28 days (-0.10〜-0.14 day^<-1>). The population change rates of genetically engineered and wild-type P. putida were not significantly different. We studied the important factors affecting the survival of genetically engineered and wild-type P. putida introduced in aquatic microcosms. Visible light exerted an adverse effect on the survival of the two strains. The densities of genetically engineered and wild-type P. putida were almost constant until 7 days after inoculation in natural water filtered with a 0.45-μm membrane filter, or treated with cycloheximide to inhibit the growth of protozoa. These results suggested that protozoan predation was one of the most important factors for the survival of two strains. We examined the impact of the addition of genetically engineered and wild-type P. putida on indigenous bacteria and protozoa. Inoculation of genetically engineered or wild-type P. putida had no apparent effect on the density of indigenous bacteria. The density of protozoa increased in microcosms inoculated with genetically engineered or wild-type P. putida at 3 days after inoculation, but after 5 to 21 days, the density of protozoa decreased to the same level as the control microcosms.
- 社団法人日本農芸化学会の論文
- 1993-08-23
著者
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Uchiyama H
National Inst. Environmental Studies Ibaraki Jpn
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Uchiyama Hiroo
National Institute Of Environmental Studies
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Uchiyama Hiroo
Water And Soil Environment Division National Institute For Enviromental Studies
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Yagi Osami
Water and Soil Environment Division, National Institute for Environmental Studies
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Yagi Osami
Water And Soil Environmental Studies
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Iwasaki K
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Yagi O
Univ. Tokyo Tokyo Jpn
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Iwasaki Kazuhiro
Regional and Community Environment Division, National Institute for Environmental Studies
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Iwasaki K
National Inst. For Environmental Studies Ibaraki Jpn
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