Immobilization of Trichloroethylene-Degrading Bacterium, Methylocystis sp. Strain M in Different Matrices
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概要
- 論文の詳細を見る
The trichloroethylene (TCE) degradation activities of a TCE-degrading methanotroph, Methylocystis sp. strain M, which was immobilized in different matrices have been investigated to identify a suitable matrix. Cells immobilized in Ca-alginate, k-carrageenan, and agarose showed higher or almost the same degradation activity in comparison with that of free cells, while low activity was observed in the cells immobilized in photocrosslinkable resin, polyurethane, and polyelectrolyte complex. In repeated use, only the agarose-immobilized cells were not damaged and retained about 40% of the initial TCE degradation activity among the cells immobilized in the three agents. The fundamental degradation characteristics of the immobilized cells were also investigated using agarose-immobilized cells. The maximum degradation rate (V_<max>) of TCE was 3.1μg of TCE/mg of dry cells・h, while the saturationconstant (K_s) was 56.5μM. The optimum pH was found to be 7.0 and the optimum temperature 35℃.
- 社団法人日本生物工学会の論文
- 1994-02-25
著者
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Uchiyama H
National Inst. Environmental Studies Ibaraki Jpn
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Uchiyama Hiroo
National Institute Of Environmental Studies
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Uchiyama Hiroo
National Institute For Environmental Studies
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Uchiyama Hiroo
Water And Soil Environment Division National Institute For Enviromental Studies
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YAGI OSAMI
National Institute of Environmental Studies
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KOKUFUTA ETSUO
Institute of Applied Biochemistry, University of Tsukuba
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OGURI KAZUHITO
Institute of Applied Biochemistry, University of Tsukuba
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Yagi Osami
Water And Soil Environmental Studies
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Yagi Osami
National Institute For Environmental Studies
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Kokufuta E
Univ. Tsukuba Ibaraki Jpn
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Kokufuta Etsuo
Institute Of Applied Biochemistry University Of Tsukuba
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Oguri Kazuhito
Institute Of Applied Biochemistry University Of Tsukuba
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Yagi O
Univ. Tokyo Tokyo Jpn
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