Development of a New Most-probable-number Method for Enumerating Methanotrophs, Using 48-well Microtiter Plates
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概要
- 論文の詳細を見る
We developed a new most-probable-number (MPN) method for enumeration of methanotrophs, using 48-well microtiter plates. With this method, a microtiter plate which has been inoculated with serial dilutions of a sample is placed in a plastic desiccator containing methane and shaken. The proposed method gave estimates of population densities of a pure culture of Methylocystis sp. strain M and of methanotrophs in a paddy soil sample similar to those obtained by a conventional MPN method using serum bottles. The new microtiter plate MPN method with an additional replica-plating technique made it possible to enumerate small populations of methanotrophs in cornfield and forest soils. It also requires less equipment, labor, and time than the conventional serum bottle MPN method.
- 日本微生物生態学会の論文
著者
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Saitoh Satoshi
Research Center for Water Environment Technology, School of Engineering, The University of Tokyo
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Saitoh Satoshi
Research Center For Water Environment Technology Graduate School Of Engineering The University Of To
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IWASAKI KAZUHIRO
National Institute for Environmental Studies
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YAGI OSAMI
Research Center for Water Environment Technology, Graduate School of Engineering, The University of
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Iwasaki K
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduat
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Yagi O
Univ. Tokyo Tokyo Jpn
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Yagi Osami
Research Center For Water Environment Technology Graduate School Of Engineering The University Of To
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Iwasaki K
National Inst. For Environmental Studies Ibaraki Jpn
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